Which class of biosafety cabinet is the most common and used for working with biological materials or organisms on campus?
Annual Biosafety Cabinet (BSC) certification:
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General recommendations:
Operational recommendations:
Frequently Asked Questions about Biological Safety Cabinets (BSCs)Who do I contact about getting a BSC? What type of BSC do I need? A Class II, A2 Biosafety Cabinet is the most common type of BSC in use as it is appropriate for most bio-hazardous work applications. Class II BSC provide personnel protection from bio-hazardous materials using HEPA filtered air prior to release into the room, in addition to providing product protection (to maintain sterility). Further information on specific classes and types of Biosafety Cabinets can be found in the CDC/NIH publication Biosafety in Microbiology and Biomedical Laboratories, 6th edition. Should my BSC be hard ducted or thimble connected to the building exhaust system? Contact OEHS (313-577-1200) before having any BSC connected (or disconnected) to the building exhaust system. Are the UV lights in my BSC effective as a secondary disinfectant? Can I use a clean bench for biohazardous work? Clean Benches may only be used for work with materials that are non-infectious. No infectious work (human cell lines, infectious microorganisms, toxins, or animal work) should be performed on a clean bench. They are not recommended for infectious work, as they only provide product protection, and in effect, blow air from the cabinet into the user's face and lap, providing no personnel protection. Why must my BSC be certified? How often must my BSC be certified? What if I need to get my BSC repaired? research.wayne.edu/oehs/forms/cabinet-service Does my BSC need to be decontaminated?. Prior to certification, all work surfaces are to be cleaned with an appropriate disinfectant before servicing. Remove all equipment, sharps and waste from the BSC. How do I get my BSC gas decontaminated? Prior to OEHS performing the gas decontamination, perform surface decontamination of all work surfaces (including grilles, drain pan, inside of sash, etc.) with an appropriate chemical disinfectant. Remove all equipment, sharps and waste from the BSC. What if I need to move or dispose of my BSC? Biosafety cabinets that are being relocated within WSU, moved out for disposal, or shipped to a separate institution require decontamination prior to being moved. In the majority of situations gas decontamination procedures will be required prior to the move. This procedure must be completed by trained WSU Biosafety Program personnel. The WSU Biosafety Program must be notified of the BSC move at least one week prior to the move date in order for a review to be completed. Submissions to the WSU Biosafety Program can be submitted using this form: research.wayne.edu/oehs/forms/cabinet-service Surface decontamination alone may be approved by the WSU Biosafety Program if both of the following conditions are met:
and,
When gas decontamination is required, it must be scheduled for a time when the lab is unoccupied, and the unit must be empty of equipment. Once decontaminated, OEH&S will provide the lab with a document certifying that the unit is ready to be moved. Users should arrange equipment moves via https://facilities.wayne.edu/design/move-management once it has been cleared by OEH&S. The moving company is used at department's expense. Do not attempt to move a BSC by yourself. Biosafety cabinets are extremely heavy and moving them without proper equipment is unsafe for personnel and can damage the BSC. Exceptions can be made for cabinets installed on mobile base stands that are being moved within a lab, or being relocated to an adjoining laboratory. After every move (even within a laboratory space) the BSC must be recertified prior to initiating work in the new location. What is the most commonly used biological safety cabinet?Class II biosafety cabinets are widely used in biological research laboratories and are differentiated into types such as A1, A2, B1, or B2. The classification for the majority of biosafety cabinets used in the United States is Class II Type A2.
Which class of biosafety cabinet is the most commonly used in animal tissue culture laboratories?Class II BSCs are the most common cabinets found in the lab and are the type of cabinet used for mammalian cell culture. Class II cabinets protect the worker, the environment and the samples. Air is HEPA-filtered as it is drawn into the cabinet and is also filtered upon exhaust.
What is a Class 1 biological safety cabinet?Class I. A Class I cabinet is defined as a ventilated cabinet for personnel and environmental protection. Class I cabinets do not offer product protection from contamination, significantly limiting their applications. They use unrecirculated airflow away from the operator.
What does a Class 2 biosafety cabinet protect?What type of protection does Class II BSC offer? Class II Biosafety Cabinets are open-fronted which protect the laboratory workers and the environment from harmful biological agents. Class II BSCs also prevent biological materials (i.e cell cultures, microbiological stocks) inside it from being contaminated.
What is class II B2 biosafety cabinet?The class II type B2 biological safety cabinets are ventilated workstations that are enclosed on three sides, have a positive pressure, and are used in microbiology labs, toxicology labs, and research labs to contain pathogens and toxic chemicals inside the work area while at the same time ensuring the samples, which ...
What is the difference between Class 1 and Class 2 biosafety cabinet?The key difference between Class I and Class II cabinets is that latter provide additional protection for the sample. The former doesn't have any minimum airflow requirements, and they can't offer the advanced exhaust system designs available with most types of Class II cabinets.
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