Listeria monocytogenes incubation temperature
Volume 11, Issues 3–4, December 1990, Pages 345-349 https://doi.org/10.1016/0168-1605(90)90028-4Get rights and content View full textEquipment and materials FDA does not specifically endorse any of commercial products listed. Equivalent products may be available.
Media and Reagents FDA does not specifically endorse any of commercial products listed. Equivalent products may be available.
Note: Alternative companies may be used when the products are equivalent. Control Cultures
Sample Preparation Sample transport and storage practices should maintain the recommended storage conditions for the food commodity. Sample analysis should be initiated as soon as possible upon sample receipt. If sample analysis must be delayed, store frozen samples frozen (-20 °C ± 5 °C); store nonperishable, canned or low-moisture foods at room temperature, and store refrigerated, unfrozen perishable foods at 4 °C ± 2 °C until sample analysis is initiated. Basic analytical options include: 1. Qualitative detection (limit of detection <1 cfu="" per="" analytical="" unit),="" 2.="" quantitative="">
The following alternative screening methodologies may be used to screen samples for the presence of Listeria. Follow the manufacturers' package insert making certain they have not deviated from the approved versions of the AOAC INTERNATIONAL Official Methods Manual protocols (Section F1). The kits are only approved for the specified food matrices, claimed in the OMA method, which vary from kit to kit. For other food matrices that are not validated a matrix extension validation is necessary. Negative results obtained with the products are considered definitive and no further testing is required. Presumptive positive results with these rapid screening methods must be confirmed by streaking to the selective agars and confirming isolates to the species level by the procedures described in sections G-I. F1. Rapid screening methods for Listeria spp. F2. Rapid screening methods for Listeria monocytogenes. Please note that these methods do not screen for Listeria spp. and therefore may not be suitable for situations in which the identification of Listeria spp. is desired.
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