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Farm production and natural forest extraction remain principal livelihood strategies of local people in many rural areas of the developing world. In this paper, we apply stochastic frontier analysis to evaluate farm production efficiency and simultaneous equations modelling to estimate the interrelationship between farm production efficiency and natural forest extraction. We use a two-year panel dataset of 430 rural households in Stung Treng province of Cambodia. We find that natural forest extraction is decreasing in farm production efficiency. Our results suggest that improving farm production efficiency, via the promotion of rural education and privatization of farm land, should be considered an integral component of natural forest conservation policy.

2.

- Title: A novel strain of porcine deltacoronavirus in Vietnam

- Journal: Arch Virol

- Author from VNUA:1. Lê Văn Phan 2. Phạm Ngọc Thạch

- Authors: Van Phan Le, Sok Song, Byung‑Hyun An, Gyu‑Nam Park, Ngoc Thach Pham, Dinh Quyen Le, Van Tam Nguyen, Thi Thu Hang Vu, Ki‑Sun Kim, SeEun Choe, Dong‑Jun An

- Abstract:

Two porcine deltacoronavirus (PDCoV) strains (Binh21 and HaNoi6) were isolated from two pig farms in North Vietnam. Phylogenetic analysis of the complete

genomes and the Spike and Membrane genes revealed that the two Vietnam PDCoVs belong to the same lineage as PDCoVs from Thailand and Laos; however, the N genes belonged to the same lineage as PDCoVs from the USA, Korea, China, and Hong Kong. The recombination detection program subsequently identified the major parent (S5011 strain) and minor parent (HKU15-44 strain) of the two Vietnam PDCoV strains (p < 0.01).

3.

- Title: Spectral monodromy of small non-selfadjoint quantum perturbations of completely integrable Hamiltonians

- Journal: Journal of Mathematical Analysis and Applications

- Author from VNUA:Phan Quang Sáng

- Authors: Phan Quang Sáng

- Abstract:

We define a monodromy, directly from the spectrum of small non-selfadjoint perturbations of a selfadjoint semiclassical operator with two degrees of freedom, which is classically integrable. It is a combinatorial invariant that obstructs globally the existence of lattice structure of the spectrum, in the semiclassical limit. Moreover this spectral monodromy allows to recover a topological invariant (the classical monodromy) of the corresponding integrable system.

4.

- Title: Degradation of β-casomorphins and identification of degradation products during yoghurt processing using liquid chromatography coupled with high resolution mass spectrometry

- Journal: Food Research International

- Author from VNUA:Nguyễn Đức Doan

- Authors: Nguyễn Đức Doan, Francesco Busetti, Stuart Johnson, Vicky Solah

- Abstract:

Liquid chromatography-high resolution mass spectrometry (LC-HRMS) was used to investigate the degradation of β-casomorphin 5 (β-CM5) and β-casomorphin 7 (β-CM7) by Streptococcus thermophilus and/or Lactobacillus delbrueckii ssp. bulgaricus, and to identify the degradation products forming during yoghurt processing. Bovine UHT milk was fermented with: (i) a single strain of L. delbrueckii ssp. bulgaricus, (ii) a single strain of S. thermophilus and (iii) the mixture of S. thermophilus and L. delbrueckii ssp. bulgaricus to pH 4.5 and then stored at 4 °C for 1 and 7 days. Results showed that L. delbrueckii ssp. bulgaricus and/or S. thermophilus completely degraded β-CM5 and β-CM7 upon fermentation to pH 4.5 and degradation products were significantly influenced by bacteria strains and storage time. Four peptides, β-CNf60–61 (YP), β-CNf62–63 (FP), β-CNf64–66 (GPI) and β-CNf62–66 (FPGPI) were tentatively identified through high resolution MS/MS experiments; however, it was not possible to confirm if either milk protein or β-casomorphins was a source releasing these peptides. Nonetheless, in this study peptides YP and GPI were released by L. delbrueckii ssp. bulgaricus. This is the first time GPI has been identified and thus future investigation of its bioactivity is warranted.

5.

- Title: Acquiring qualified samples for RANSAC using geometrical constraints

- Journal: Pattern Recognition Letters, Elsevier

- Author from VNUA:Nguyễn Thị Thủy (CNTT)

- Authors: Van-Hung Le, Hai Vu, Thi-Thuy Nguyen, Thi-Lan Le, Thanh-Hai Tran

- Abstract:

Estimating parameters of a geometrical model from 3-D point cloud data is an important problem in computer vision. Random sample consensus (RANSAC) and its variations have been proposed for the estimation of the models parameters. However, RANSAC is computationally expensive and the problem is challenging when the measured 3-D data contain noise and outliers. This paper presents an efficient sampling technique for RANSAC, in which geometrical constraints are utilized for selecting good samples for a robust estimation. The constraints are based on two predefined criteria. First, the samples must ensure being consistent with the estimated model; second, the selected samples must satisfy explicit geometrical constraints of the interested objects. The proposed approach is wrapped as a robust estimator, named GCSAC (Geometrical Constraint SAmple Consensus), for estimating a cylindrical object from a 3-D point cloud. Extensive experiments on various data sets show that our method outperforms other robust estimators (e.g. MLESAC) tested in term of both precision of the estimated model and computational time. The implementations and evaluation datasets used in this paper are made publicly available.

6.

- Title: A lytic bacteriophage of the newly emerging rainbow trout pathogen Weissella ceti

- Journal: Virus Research

- Author from VNUA:Trương Đình Hoài

- Authors: Truong Dinh Hoai, Kyoka Mitomi, Issei Nishiki, Terutoyo Yoshida

- Abstract:

This study was conducted to isolate and characterize a bacteriophage of a newly emerging pathogen, Weissella ceti, which causes weissellosis outbreaks of intensively farmed rainbow trout worldwide. The phage appeared together with the cultured Weissella ceti during isolation of pathogen from kidney of diseased rainbow trout. The morphological, physiological, proteomic and lytic spectrum were characterized. This phage, named PWc, belonged to the family Siphoviridae and possessed an isometric head (approximately 65 nm in diameter) and a flexible, non-contractile tail of 170–180 nm in length. The latent time and burst size of PWc were approximately 25 min and 16 PFU/infected cells, respectively. The PWc was relatively stable over a wide range of temperatures and pH values and possessed a broad lytic spectrum, lysing all 36 tested W. ceti strains isolated from diseased rainbow trout in Japan. The protein profile of the phage was obtained using SDS-PAGE analysis, and the potential packaging strategy was determined based on terminase large subunit sequence analysis. This is the first study to investigate a lytic bacteriophage of a newly emerging pathogen W. ceti that causes infectious disease in rainbow trout.

7.

- Title: First isolation of Weissella ceti responsible for outbreaks of weissellosis in farmed rainbow trout in Japan

- Journal: Journal of Fish Diseases

- Author from VNUA:Trương Đình Hoài

- Authors: Truong Dinh Hoai, Kyoka Mitomi, Issei Nishiki, Terutoyo Yoshida

- Abstract:Disease of rainbow trout

8.

- Title: Characterization and Genetic Structure of a Tospovirus Causing Chlorotic Ring Spots and Chlorosis Disease on Peanut; Comparison with Iranian and Polish Populations of Tomato yellow fruit ring virus

- Journal: Plant Disease

- Author from VNUA:Nguyễn Đức Huy

- Authors: Ali Reza Golnaraghi, Nooh Shahraeen, H. D. Nguyen

- Abstract:

A Tospovirus was isolated from peanut plants showing chlorotic ring spots and chlorosis, and identified as Tomato yellow fruit ring virus (TYFRV) on the basis of its biological, serological and molecular properties. In host range studies, a broad range of indicator plants was infected by the five isolates studied; all the isolates systemically infected Nicotiana tabacum cultivars, and so, they were classified into the N host-infecting type isolates of the virus. These isolates strongly reacted with TYFRV antibodies, but not with the specific antibodies of other tospoviruses tested. Recombination analyses showed that the nucleoprotein gene of the peanut isolates and other isolates studied were non-recombinant. In phylogenetic trees, the virus isolates were clustered in three genogroups: IRN-1, IRN-2 and a new group, namely POL; the peanut isolates fell into IRN-2 group. Multiple sequence alignments showed some genogroup-specific amino acid substitutions among the virus isolates studied. The results revealed the presence of negative selection in TYFRV populations. Also, the Iranian populations had higher nucleotide diversity as compared with the Polish population. Genetic differentiation and gene flow analyses indicated that the populations from Iran and Poland, and those belonging to different genogroups were partial differentiated populations. Our findings seem to suggest that there has been frequent gene flow between some populations of the virus in the mid-Eurasia of Iran.

9.

- Title: STAT6, PBX2, and PBRM1 Emerge as Predicted Regulators of 452 Differentially Expressed Genes Associated With Puberty in Brahman Heifers

- Journal: Frontiers in Genetics

- Author from VNUA:Nguyễn Tố Loan

- Authors: Loan T. Nguyen, Antonio Reverter, Angela Cánovas, Bronwyn Venus, Stephen T. Anderson, Alma Islas-Trejo, Marina M. Dias, Natalie F. Crawford, Sigrid A. Lehnert, Juan F. Medrano, Milt G. Thomas, Stephen S. Moore and Marina R. S. Fortes

- Abstract:

The liver plays a central role in metabolism and produces important hormones. Hepatic estrogen receptors and the release of insulin-like growth factor 1 (IGF1) are critical links between liver function and the reproductive system. However, the role of liver in pubertal development is not fully understood. To explore this question, we applied transcriptomic analyses to liver samples of pre- and post-pubertal Brahman heifers and identified differentially expressed (DE) genes and genes encoding transcription factors (TFs). Differential expression of genes suggests potential biological mechanisms and pathways linking liver function to puberty. The analyses identified 452 DE genes and 82 TF with significant contribution to differential gene expression by using a regulatory impact factor metric. Brain-derived neurotrophic factor was observed as the most down-regulated gene (P = 0.003) in post-pubertal heifers and we propose this gene influences pubertal development in Brahman heifers. Additionally, co-expression network analysis provided evidence for three TF as key regulators of liver function during pubertal development: the signal transducer and activator of transcription 6, PBX homeobox 2, and polybromo 1. Pathway enrichment analysis identified transforming growth factor-beta and Wnt signaling pathways as significant annotation terms for the list of DE genes and TF in the co-expression network. Molecular information regarding genes and pathways described in this work are important to further our understanding of puberty onset in Brahman heifers.

10.

- Title: Transcription analysis of the ethylene receptor and CTR genes in tomato: The effects of on and off-vine ripening and 1-MCP

- Journal: Postharvest Biology and Technology

- Author from VNUA:Trần Thị Định

- Authors: Clara I.Mata, Bram Van de Poel, Maarten Hertog, Dinh T. Tran, Bart Nicolaï

- Abstract:Transcription analysis of the ethylene receptor and CTR genes in tomato: The effects of on and off-vine ripening and 1-MCP

11.

- Title: Enhancement of immune response and resistance against Vibrio parahaemolyticus in kuruma shrimp (Marsupenaeus japonicus) by dietary supplementation of β-1,4-mannobiose

- Journal: Fish & Shellfish Immunology Volume Issue Pages Advanced searchnew

- Author from VNUA:Vũ Đức Hạnh

- Authors: Gehad E.Elshopakey, Engy F.Risha, Osama A.Abdall, YoOkamura, Vu DucHanh, MasahisaIbukie, RajaSudhakaran, ToshiakiItami

- Abstract:

Abstract

Supplementation of prebiotic carbohydrates can act as a potent immunomodulator and have the efficacy to induce immune-related genes which are involved in host defense. Pure β-1,4-mannobiose (MNB) showed activation of prophenoloxidase system of shrimp hemocytes in vitro. The resistance of kuruma shrimp Marsupenaeus japonicus against Vibrio parahaemolyticus was examined after the shrimp were fed with 0 (control), 0.02, 0.2, and 2% MNB supplemented diets. The results showed significantly higher survival rates in MNB supplemented shrimp than those of the control one from 2 to 12 days post challenge. In another experiment, the hemocyte count, ROS production, phagocytic, phenoloxidase and bactericidal activities, and expression of immune-related genes were investigated in the control and MNB supplemented groups at day 1, 4, 6, 8 and 11 of the feeding. These immune parameters were significantly enhanced in MNB supplemented groups. Furthermore, the gene expression analysis showed that transcripts of lysozyme, crustin, penaeidin and TNF were significantly up-regulated in hemolymph, lymphoid organs and intestines of MNB treated shrimp. Overall, the results provided evidence that MNB supplementation could improve the immune response and increase shrimp resistance against V. parahaemolyticus infection.

12.

- Title: Host range and the influence of a cell capsule on the phage efficacy of three Lactococcus garvieae lytic phages

- Journal: Diseases of Aquatic Organisms

- Author from VNUA:Trương Đình Hoài

- Authors: Truong Dinh Hoai, Issei Nishiki, Terutoyo Yoshida, Toshihiro Nakai

- Abstract:

13.

- Title: Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

- Journal: Archives of Gene and Genome Research

- Author from VNUA:1. Nguyễn Văn Giáp 2. Huỳnh Thị Mỹ Lệ 3. Cao Thị Bích Phượng 4. Lê Văn Trường 5. Vũ Thị Ngọc

- Authors: Van Giap Nguyen, Hee Chun Chung, Thi My Le Huynh, Thi Bich Phuong Cao, Thi Ngoc Vu, Van Truong Le, Hong Quan Pham

- Abstract:

In 2015, a novel circovirus (Porcine circovirus 3, PCV3) was detected for the first time from pigs suffered from porcine dermatitis and nephropathy syndrome and reproductive failure. Since then, PCV3 has been reported in several pig producing countries. This study was carried out in order to investigate the presence and further genetic characterization of PCV3 in the pig populations in northern Vietnam. The screening PCR detected the presence of PCV3 in 6 out of 135 samples (4.44%) which were collected from seven northern provinces in 2011 and 2016-2017. The capsid-coding gene (ORF2, 645 nucleotides in length) was successfully sequenced from 5 out of 6 field strains. Compared to a highly diverse PCV3 strain (GD2016-1, KY421347) 5 Vietnamese PCV3 strains contained 39 point nucleotide mutations and 9 of those were non-synonymous. The Bayesian phylogenetic analysis on the basic of ORF2 revealed that PCV3 evolved at a comparable evolutionary rate of the pathogenic PCV2 (2.284 × 10-3 and 1.440 × 10-3, respectively). Besides, this analysis suggested PCV3 could be separated into PCV3a and PCV3b groups, of which the majority of Vietnamese PCV3 strains belong to PCV3a (sub-cluster a1).

14.

- Title: Towards global applicability? Erosion source discrimination across catchments using compound-specific δ13 C isotopes

- Journal: Agriculture, Ecosystems and Environment

- Author from VNUA:Nguyễn Thanh Lâm

- Authors: Christian Brandt, Gerd Derconb, Georg Cadisch, Làm T. Nguyễn, Paulina Schuller, Claudio Bravo Linares, Alejandra Castillo Santana, Valentin Golosove, Moncef Benmansour, Nourredine Amenzoug, Zhang Xinbaoh, Frank Rasche

- Abstract:

Accurate identification of soil erosion hot spots across catchments of different sizes and agro-ecologies through the use of conventional tracing techniques has proven challenging. Since this problem hinders implementation of precise soil conservation measures by land managers and decision-making bodies, novel evidence-based techniques are needed. To meet this need, the International Atomic Energy Agency (IAEA, Vienna, Austria) initiated the Coordinated Research Project entitled “Integrated Isotopic Approaches for an Area-wide Precision Conservation to Control the Impacts of Agricultural Practices on Land Degradation and Soil Erosion – D1.20.11” in 2008. This project emphasized the application of isotopic approaches to identify hot spots of land degradation

in agricultural catchments to develop effective soil conservation measures. As one key outcome of this project, we present here an aligned protocol to explore the potential of compound-specific stable isotope (CSSI) analysis of individual fatty acids (FA) combined with Bayesian statistics to discriminate sediment sources across six catchments of different sizes and agro-ecologies. The global applicability of the CSSI approach was first tested on the basis of major land use categories (i.e., forest, cultivated and non-cultivated land) as potential sediment sources in the studied catchments. These land use categories were then further resolved into specific land use types (e.g., cassava and maize fields, orchards) to assess the potential resolution threshold of the CSSI technique.

In a final step, the influence of miscellaneous sources (e.g., roads, channel banks) that had the potential to distort proportional contributions to sediment deposition was assessed. The introduced aligned protocol of the CSSI technique was applied to discriminate individual erosion sources based on land use types of tested catchments. Merging catchment-specific land use types with broader land use categories made it possible to determine intercatchment comparisons of isotopic signatures due to significant differences in δ13C values of fatty acids when cultivated land was present. Notably, no correlations were found between different catchment sizes, agro-ecologies, number and type of land use types, or soil organic carbon concentrations and the number of significant δ13C fatty acid values of the various land use types. Thus, we propose that the presented CSSI technique has the potential to identify soil erosion hot spots in contrasting catchments of different sizes and agro-ecologies.

15.

- Title: Integrating compound-specific δ13 C isotopes and fallout radionuclides to retrace land use type-specific net erosion rates in a small tropical catchment exposed to intense land use change

- Journal: Geoderma

- Author from VNUA:Nguyễn Thanh Lâm

- Authors: Christian Brandt, Moncef Benmansour, Leander Walza, Lam T. Nguyen, Georg Cadischa, Frank Raschea

- Abstract:

Retracing net erosion rates linked to land use change in tropical agricultural catchments dominated by smallholder farmers is challenging, due largely to catchment heterogeneity and uncontrolled farming practices. To tackle this problem and to complement a preceding study (Brandt et al., 2016), we introduce here an advanced approach that integrates compound-specific δ13C isotopes (CSSI) and fallout radionuclides (FRN), Excess lead-210 (210Pbex) and Cesium-137 (137Cs) to estimate past net erosion rates of dominant land use types in themountainous catchment Chieng Khoi (207 ha, Northwest Vietnam). Spatially-integrated topsoil (0 to 2 cm)samples of dominant land use types (e.g., protected and secondary forests, teak, fruit orchards, maize, cassava)were collected from at least three discrete plots of each land use type within the upland area (i.e., erosion sites)of the studied catchment. In the corresponding lowland area, a representative sediment profile was localized and divided into sections of 2 to 4 cm for CSSI and FRN analysis. Samples for FRN reference data were taken from undisturbed areas in close proximity. At a soil deposition site near a lakeshore,

210 Pb ex data determined the age and sediment accumulation rates of 19 sediment layers to a depth of 38 cm. Based on 210 Pbex activity, maximum

sediment accumulation rates of 127 t ha−1 y−1 were calculated, corresponding to erosion rates of about 16 t ha−1 y−1

for the total catchment area. CSSI data confirmed that maize and cassava were the most important erosion sources during a period of dramatic land use change (1987 to 2004), when forests were cleared and high-yielding maize hybrids were introduced. Based on integrated FRN and CSSI data, net erosion rates of maize and cassava reached maximum rates of 4.8 t ha−1, y−1, (maize), 6.2 t ha−1, y−1 (cassava). This major finding verified the potential of integrating FRN and CSSI to accurately estimate land use type-specific net erosion rates. In conclusion, determining past sediment budgets for specific land use types provides insight into the accelerating impact of specific land use change on soil retrogression and degradation. Such knowledge is of prime importance for effective soil conservation through evidence-based land management and decision making.

16.

- Title: Identification of drought-induced genes in giant leucaena (Leucaena leucocephala subsp. glabrata)

- Journal: Trees- Springer

- Author from VNUA:Phạm Thị Dung

- Authors: Michael D. H. Honda · Kazue L. Ishihara · Dung T. Pham· Dulal Borthakur

- Abstract:

Abstract Leucaena leucocephala subspecies glabrata (leucaena), which is also known as ‘giant leucaena’, is a multipurpose tree legume that can be grown and maintained as a woody tree for wood and paper pulp production, or as a shrubby legume through repeated harvest of foliage for use as a fodder. Leucaena is naturally resistant to most biotic and abiotic stresses including drought. The objective of this research was to identify drought-responsive genes in leucaena seedlings through microarray and qRT-PCR analyses. Ten thousand cDNA sequences selected from a leucaena transcriptome were screened for higher expression in a 4 × 180 k microarray using RNA isolated from drought-treated and untreated leucaena seedlings. Microarray analysis identi ed 73 and 39 drought-responsive genes sequences that were upregulated in the root and shoot, respectively. qRT-PCR analyses con rmed the root- and shoot-speci c upregulation of some of the most highly expressed gene sequences identi ed in the microarray analysis. The proteins encoded by these genes could be grouped into four classes: (1) receptors that sense osmotic and oxidative stresses, (2) transcription factors, (3) degradation pathway enzymes, and (4) biosynthetic enzymes and structural proteins. The functions of these four classes of proteins can be used to explain the drought response in leucaena. The NB-LRR class of disease-resistance proteins may serve as receptors for detecting osmotic and oxi- dative stresses induced by drought, leading to induction of transcription factors such as zinc nger CCCH domain-containing protein and TCP14. As result, the genes for some degradative pathway enzymes, such as GDSL esterase/lipase, lipid acyl hydrolase patatin, and serine carboxypeptidase, were induced. Similarly, the expression of certain biosynthetic enzymes and structural proteins such as avonoid 3-O-galactosyl transferase, ca eoyl-CoA O-methyltransferase, 9-cis-epoxy carotenoid dioxygenase, β-amyrin synthase, pentatricopeptide-containing protein, GPI-anchored protein COBRA, and peroxin, were also induced. High expression of some of these drought-inducible genes in leucaena seedlings may be used as a selection criterion in future breeding programs for drought resistance in leucaena and other legumes.

17.

- Title: Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus)

- Journal: Aquaculture

- Author from VNUA:Kim Văn Vạn

- Authors: Hien Van Doan,, Seyed Hossein Hoseinifar, Chartchai Khanongnuch, Apinun Kanpiengjai, Kridsada Unban, Van Van Kim, Satawat Srichaiyo

- Abstract:

The investigation was conducted to evaluate the possible effects of singular or combined administration of hostassociated probiotics (Lactobacillus plantarum N11 and Bacillus velezensis H3.1) on several innate-immune parameters, disease resistance, and growth performance of Nile tilapia, (O. niloticus). Tilapia fingerlings were assigned for te following diets: Diet 1 (0 - control), Diet 2 (107 CFU g−1 B. velezensis H3.1), Diet 3 (108 CFU g−1 L.

plantarum N11), and Diet 4 (107 CFU g−1 B. velezensis H3.1 + 108 CFU g−1 L. plantarum N11). At days 15 and 30 post feeding, skin mucus and innate immune parameters as well as growth performance were collected. The challenge test was conducted at the end of feeding trial (i.e 30 days post feeding). The results showed that dietary administration of Lactobacillus plantarum N11 and Bacillus velezensis H3.1 resulted in significant increase skin mucus lysozyme and peroxidase activities of the fish (P < 0.05). However, no significant difference was detected among supplemented groups (P > 0.05). For serum immunity, serum lysozyme activity of fish fed probiotics diets was significantly higher than the control group, except for fish fed B. velezensis H3.1, where no significant was observed when compared with the control (P > 0.05). Likewise, serum peroxidase, alternative complement, phagocytosis, and respiratory burst activities were significantly higher in treated groups compared to the control (P < 0.05). Also, the relative percent of survival (RPS) in L. plantarum N11 and B. velezensis H3.1, L. plantarum N11, and B. velezensis H3.1 treatments were 58.33%, 54.17%, 41.67%, respectively. Nonetheless, no significant differences were observed among supplemented groups regarding RPS (P > 0.05). However, dietary administration of host-associated probiotics remarkably improved the growth performance, specific growth rate, weight gain, final weight, and feed conversion ratio (P < 0.05). The present results indicated that singular or combined administration Lactobacillus plantarum N11 and Bacillus velezensis H3.1 can be considered as beneficial feed additives for Nile tilapia.

18.

- Title: Molecular characterization of US-like and Asian non-S INDEL strains of porcine epidemic diarrhea virus (PEDV) that circulated in Japan during 2013–2016 and PEDVs collected from recurrent outbreaks

- Journal: BMC Veterinary Research

- Author from VNUA:Nguyễn Văn Điệp

- Authors: Nguyen Van Diep, Masuo Sueyoshi, Junzo Norimine, Takuya Hirai, Ohnmar Myint, Angeline Ping Ping Teh, Uda Zahli Izzati, Naoyuki Fuke, and Ryoji Yamaguchi

- Abstract:

Since late 2013, porcine epidemic diarrhea virus (PEDV) has reemerged in Japan and caused severe economic losses to the swine industry. Although PEDV vaccines have been used widely, the disease has swept rapidly across the county, and is commonly observed in PED-vaccinated farms, and has recurred in domestic herds. To better understand PEDVs responsible for the reemerging outbreaks in Japan, full-length spike (S), membrane (M), and nucleocapsid (N) genes of 45 PEDVs collected in Japan during 2013–2016, were sequenced and analyzed.

Results

Phylogenetic analysis based on S gene sequences revealed that all the recent field PEDVs were genetically distinct from the classical Japanese strains, and were classified into three genotypes: North American (NA), S INDEL, and Asian non-S INDEL. Our data suggested a possibility that multiple parental PEDV strains were introduced into Japan from abroad at the same time or similar times. The newly identified Japanese strains showed the closest relationship to the US strains. Two sublineages of Japanese strains circulating in Japan were similar to two sublineages identified in the US, suggesting common ancestors for these strains. In comparison with two vaccine strains used in Japan, the field strains had various changes in epitope regions, glycosylation sites, and phosphorylation sites. These substitutions, particularly observed in epitope regions of the S (521, 553, 568, and 570), M (5), and N (123, 252, and 255) proteins, may have affected antigenicity and vaccine efficacy, resulting in an unsuccessful PEDV control. Sequence comparisons between PEDVs collected from primary and secondary outbreaks in three herds revealed that the disease has developed to an endemic stage in which PEDV could persist for nearly two years in the herds or local regions, causing subsequent epidemics.

Conclusions

These results elucidate the genetic characteristics, origin, and molecular epidemiology of PEDVs circulating in Japan, as well as the PEDV strains causing recurrent outbreaks. This study provides a better insight into the PEDVs responsible for recent outbreaks in Japan, and could potentially help to develop measures for controlling and preventing the disease.

19.

- Title: Merkel cell carcinoma in a Steer

- Journal: Journal of Comparative Pathology

- Author from VNUA:Nguyễn Văn Điệp

- Authors: Angeline Ping Ping Teh, Uda Zahli Izzati, Nguyen Van Diep, Takuya Hirai,

Naoyuki Fuke, and Ryoji Yamaguchi

- Abstract:

Merkel cell carcinoma is a rare and aggressive cutaneous neuroendocrine tumour reported only in man, dogs and cats. A 20-month-old Japanese black fattening steer was presented with necrotic protruding skin masses over the left thoracic area and a 20 25 cm subcutaneous mass in the left abdominal area. Microscopical evaluation of the masses revealed cords of small to medium-sized round tumour cells with marked anisocytosis and anisokaryosis and clear and vacuolated cytoplasm, which were separated by a delicate fibrovascular stroma and arranged in a trabecular and nested pattern. Necropsy examination revealed multiple solid white nodular masses in the lungs. Immunohistochemistry (IHC) for cytokeratin (CK) 20 and CKAE1/3 revealed focal perinuclear labelling of tumour cells. IHC for the neuroendocrine markers chromogranin A and neuron specific enolase, the neuroepithelial stem cell marker nestin and the hormonal markers adrenocorticotropic hormone and calcitonin revealed diffuse cytoplasmic labelling of all tumour cells. Ultrastructurally, the tumour cells contained few neurosecretory granules and abundant glycogen pools. The tumours were diagnosed as Merkel cell carcinoma with pulmonary metastases and this case represents the first such diagnosis in cattle.

20.

- Title: Land Surface Temperature Variation Due to Changes in Elevation in Northwest Vietnam

- Journal: Climate, volume 6, issue 2

- Author from VNUA:1. Trần Trọng Phương 2. Phan Thành Nội

- Authors: Thanh Noi Phan(1), Martin Kappas and Trong Phuong Tran(1)

- Abstract:

Land surface temperature (LST) is one of the most important variables for applications relating to the physics of land surface processes. LST rapidly changes in both space and time, and knowledge of LST and its spatiotemporal variation is essential to understand the interactions between human activity and the environment

21.

- Title: Isolation and application of bacteriophages to reduce Salmonella contamination in raw chicken meat

- Journal: LWT-Food Science and Technology

- Author from VNUA:1. Hoàng Minh Đức 2. Hoàng Minh Sơn

- Authors: Hoang Minh Duc, Hoang Minh Son, Ken-ichi Honjoh, Takahisa Miyamoto

- Abstract:

Chicken meats are considered as main sources associated with Salmonella infections in humans. In this study, lytic phages against Salmonella were isolated and examined for their efficacy to control Salmonella. Eighteen lytic phages were isolated from raw chicken skin and gizzard. Five phages belonging to Myoviridae and Siphoviridae families were characterized and selected for bacterial challenge tests. The treatment of raw chicken breast samples contaminated with S. Enteritidis and S. Typhimurium at 8 °C by the cocktail of five phages significantly reduced (P < 0.05) viable counts by 1.41 and 1.86 log CFU/piece, respectively. When incubated at 25 °C, the highest reductions of viable counts of S. Enteritidis and S. Typhimurium in the phage-treated samples were 3.06 and 2.21 log CFU/piece, respectively (P < 0.05). These data suggested that the phages isolated from raw chicken meats are potential agents for controlling Salmonella in raw meats.

22.

- Title: Genotypic variations in the plasticity of nodal root penetration through the hardpan during soil moisture fluctuations among four rice varieties.

- Journal: Plant Production Science

- Author from VNUA:Nguyễn Thị Ngọc Dinh

- Authors: Dinh Thi Ngoc Nguyena, Roel Rodriguez Suraltab, Mana Kano-Nakataa,c, Shiro Mitsuyaa, Stella Owusu-Nketiaa and Akira Yamauchia

aGraduate School of Bioagricultural Sciences, nagoya university, nagoya, Japan; bagronomy, Soils and Plant Physiology division,

- Abstract:

Rainfed lowland rice fields are characterized by soil moisture fluctuations (SMF) and the presence of hardpan that impedes deep rooting and thus limits water extraction from deep soil layer during the periods of drought. In this study, we used rootboxes with three layers; shallow layer, artificial hardpan, and deep and wet layer below the hardpan, to evaluate differences in the plasticity of nodal roots elongation through the hardpan and promote root branching below the hardpan in response to SMF among four rice varieties; Sasanishiki, Habataki, Nipponbare and Kasalath. Experiments were conducted during the summer and autumn seasons. Plasticity was computed as the difference in root traits within each variety between the SMF and continuously well-watered treatments. In both experiments, Habataki consistently tended to exhibit higher root plasticity than the other three varieties by increasing number of nodal roots that penetrated the hardpan during rewatering period in SMF, when the soil moisture increased and penetration resistance decreased. This root plasticity then contributed to greater water use at the deeper soil during the subsequent drought period and overall shoot dry matter production. Habataki had significantly higher δ13C value in roots at deep layer than roots at the shallow and hardpan layers under SMF, which may indicate that these were relatively newly grown roots as a consequence of root plasticity. This study also indicates that CSSLs derived from Sasanishiki and Habataki varieties may be suitable for the analysis of QTLs associated with root plasticity expression in rainfed lowland with hardpan and experiencing SMF.

23.

- Title: Root plasticity under fluctuating soil moisture stress exhibited by backcross inbred line of a rice variety, Nipponbare carrying introgressed segments from KDML105 and detection of the associated QTLs

- Journal: Plant Production Science Vol 21 Issue 2

- Author from VNUA:Nguyễn Thị Ngọc Dinh

- Authors: Stella Owusu-Nketiaa, Yoshiaki Inukaib, Satomi Ohashia, Roel Rodriguez Suraltac, Kazuyuki Doia, Shiro Mitsuyaa, Mana Kano-Nakataa,d, Jonathan Manito Nionese, Dinh Thi Ngoc Nguyena,f, Kabuki Takuyaa, Daigo Makiharab and Akira Yamauchia a Graduate School of

- Abstract:

In rainfed lowland rice ecosystem, rice plants are often exposed to alternating recurrences of waterlogging and drought due to erratic rainfall. Such soil moisture fluctuation (SMF) which is completely different from simple or progressive drought could be stressful for plant growth, thereby causing reduction in yield. Root plasticity is one of the key traits that play important roles for plant adaptation under such conditions. This study aimed to evaluate root plasticity expression and its functional roles in dry matter production and yield under SMF using Nipponbare, KDML 105 and three backcross inbred lines (BILs) and to identify QTL(s) associated with root traits in response to SMF at two growth stages using Nipponbare/KDML105 F2 plants. A BIL, G3-3 showed higher shoot dry matter production and yield than Nipponbare due to its greater ability to maintain stomatal conductance concomitant with greater root system development caused by promoted production of nodal and lateral roots under SMF. QTLs were identified for total nodal root length, total lateral root length, total root length, number of nodal roots, and branching index under SMF at vegetative and reproductive stages. The QTLs detected at vegetative and reproductive stages were different. We discuss here that relationship between root system of G3-3 and the detected QTLs. Therefore, G3-3 and the identified QTLs could be useful genetic materials in breeding program for improving the adaptation of rice plants in target rainfed lowland areas.

24.

- Title: Laws, Regulations, Guidelines, and Principles Pertaining to Laboratory Animals in Southeast Asia

- Journal: Laboratory Animals (Second Edition) Regulations and Recommendations for the Care and Use of Animals in Research 2018

- Author from VNUA:Nguyễn Bá Tiếp

- Authors: Montip Gettayacamin,1, Richard Grant2, Holl Davun3, John E. Heidrich4, Sorn San5, Yasmina Arditi Paramastri6, Imelda Liunanita Winoto7, Dondin Sajuthi7, Goh Yong Meng8, Abdul Rahim Mutalib8, Ranillo Rodrigo G. Resuello9, Maria Amelita C. Estacio10, Joel V

- Abstract:

This article describes current laws, regulations, guidelines, and principles pertaining to animals used for research, teaching, and testing in some countries of Southeast Asia region including Cambodia, Indonesia, Malaysia, Philippines, Singapore, Thailand, and Vietnam. There is no specific overarching legislation applied for animal welfare relevant directly to animals used for research, testing, and teaching in the region. All countries in the Southeast Asia subregion are members of the World Organisation for Animal Health (OIE). The OIE Terrestrial Animal Health Code (the Terrestrial Code) sets out standards for the improvement of animal health and welfare and veterinary public health worldwide, including standards for safe international trade in terrestrial animals (mammals, birds, and bees) and their products. This is achieved through the detailing of animal health measures to be used by the veterinary authorities of importing and exporting countries to avoid the transfer of agents pathogenic for animals or humans, while avoiding unjustified trade barriers. In 2015, there was the publication of the Animal Welfare Act by the parliament of Malaysia and the Animals for Scientific Purposes Act by the Royal Thai Government. Recently, the Veterinary Law has come into effect in July 2016 marking a progress in animal welfare in Vietnam. There has been no recent development of any law pertaining to laboratory animals in Cambodia.

25.

- Title: Plasticity in nodal root elongation through the hardpan triggered by rewatering during soil moisture fluctuation stress in rice

- Journal: scientific reports

- Author from VNUA:Trần Thị Thiêm

- Authors: Roel Rodriguez Suralta, Jonathan Manito Niones, Mana Kano-Nakata, Thiem Thi Tran, Shiro Mitsuya & Akira Yamauchi

- Abstract:

Rainfed lowland (RFL) rice fields have hardpans and experience soil moisture fluctuations (SMF) stress, which influence root system development. Here, we clarify the expression and timing of the plasticity in nodal root elongation through the hardpan under SMF and its contribution to shoot growth using a shallow-rooting IR64 and its deep-rooting introgression line, YTH304. Under SMF, soil moisture content had negative relationship with soil penetration resistance, regardless of hardpan bulk densities. YTH304 had greater root system below the hardpan than IR64 in hardpan with 1.50 but not in 1.70 g cm−3 bulk density (BD). YTH304 had greater plasticity in nodal root elongation through the hardpan than IR64 under SMF, which was clearly expressed during rewatering. YTH304 also had greater soil water uptake below the hardpan during drought and greater shoot growth than IR64. The results imply that deep root system development during SMF was due to the plasticity in nodal root elongation through the hardpan expressed during rewatering rather than during drought periods. This is against the long standing belief that active root elongation through the hardpan happens during drought. This also implies a need to revisit current root screening methods to identify rice lines with good hardpan penetration ability.

26.

- Title: By-product Originating from Artisan Distillers of Rice Alcohol in Northern Vietnam: Production, Use and Nutrient Value for Smallholder Pig Raising

- Journal: World Journal of Agricultural Research

- Author from VNUA:1. Nguyễn Công Oánh 2. Phạm Kim Đăng 3. Đỗ Đức Lực 4. Vũ Đình Tôn

- Authors: Nguyen Cong Oanh, Pham Kim Dang, Do Duc Luc, Jérôme Bindelle, Nassim Moula, Vu Dinh Ton, Jean-Luc Hornick

- Abstract:

The objective of this study was to investigate by-product production originating from artisan distillers of rice alcohol and its use as pig feed in three provinces (Hai Duong, Hung Yen and Bac Giang) of Northern Vietnam. A total of 120 rice alcohol producers classified by production scales (30 producers for Large-L, 45 producers for Medium-M and 45 producers for Small-S) were interviewed from January to August 2015. Additionally, sixty-three rice distiller’s by-product samples were collected from the study areas to determine their daily nutrient content according to one week storage time. The annual dry matter feedstuff production was estimated to 4.8, 3.0 and 2.1 tons per household for L, M and S scales, respectively (p<0.001). Swine herd size increased with the scale of household alcohol production (p<0.05). The proportion of rice distiller’s by-product in diet of swine lowered when turning from pregnant to lactating sows, and varied in fattening pigs. Rice distiller’s by-product was rich in high quality crude protein, copper and lactic acid but poor in dry matter. Its nutritive value remained stable over a week under ambient conditions. In conclusion, rice distiller’s by-product provides significant and stable amounts of nutrients in northern vietnamese pig production

27.

- Title: SCREENING ANTIBACTERIAL EFFECTS OF VIETNAMESE PLANT EXTRACTS AGAINST PATHOGENS CAUSED ACUTE HEPATOPANCREATIC NECROSIS DISEASE IN SHRIMPS

- Journal: Asian Journal of Pharmaceutical and Clinical Research

- Author from VNUA:1. Nguyễn Thanh Hải (CNSH) 2. Nguyễn Thị Thanh Hà ( TY) 3. Hoàng Hải Hà 4. Lại Thị Ngọc Hà

- Authors: Hai Thanh Nguyen, Lua Thi Dang, Hanh Thi Nguyen, Hai Ha Hoang, Ha Thi Ngoc Lai, Ha Thi Thanh Nguyen

- Abstract:

ABSTRACT

Objectives: The objectives are aimed to investigate the antibacterial properties of five Vietnamese medicinal plants against acute hepatopancreatic

necrosis disease (AHPND)-caused bacterial pathogens, to verify their potentials to apply as a new treatment therapy.

Methods: Extracts from plants, such as Psidium guajava leaf, Piper betle L. leaf, Phyllanthus amarus leaf, Rhodomyrtus tomentosa seed, and Allium sativum bulb, were tested against three AHPND-caused bacteria. Agar infusion and broth dilution methods were employed to evaluate extract in vitro antibacterial effects, while experiments with cultured whiteleg shrimps were applied to access their safety when applied in vivo. High-performance

liquid chromatography (HPLC) analysis was applied to identify components in the extracts.

Results: P. amanus and R. tomentosa extracts exerted the strongest inhibition on tested bacteria. Other extracts, including P. betel and P. guajava, were less effective, while A. sativum showed no effects against bacteria. In safety assessment experiments, we observed that only crude extracts of R. tomentosa and A. satium were safe, while others significantly reduced their survival rates. HPLC showed that extracts of high antibacterial

properties had rich phenol constituents. In addition, the phenolic profile of R. tomentosa showed the presence of piceatannol.

Conclusion: Considering both of antibacterial effects and safety properties altogether, we concluded that among the five examined plant materials of this study, R. tomentosa had the highest potential to apply in AHPND treatment, as only this plant showed the high effects on pathogenic bacteria while were still safe for host aquatic shrimps.

Keywords: Medicinal plant, Rhodomyrtus tomentosa, Antibacterial effect, Acute hepatopancreatic necrosis disease, Acute hepatopancreatic necrosis disease, Shrimp

28.

- Title: Phylogenetic position of aerophytic Cyanophyta/Cyanobacteria, the genus Porphyrosiphon Kützing ex Gomont (Oscillatoriales, Microcoleaceae) from Nakaoi Valley, Kochi Prefecture, Japan

- Journal: Phycological Research

- Author from VNUA:Nguyễn Xuân Hòa

- Authors: Fukuoka Masayuki, Xuan Hoa Nguyen, Suzuki Hidekazu, Tanaka Jiro, Suda Shoichiro

- Abstract:

One sample was collected from Kochi Prefecture and we established a culture strain. Morphologically, the sample was identified as the type species of Porphyrosiphon, P. notarisii (LC331913), and our phylogenetic results showed the strain has no closely related cyanobacteria relatives.

29.

- Title: Farmers’ adoption of organic production

- Journal: Asia-Pacific Journal of Regional Science

- Author from VNUA:Tô Thế Nguyên

- Authors: Lê Văn Cường và Tô Thế Nguyên

- Abstract:

This paper presents a theoretical model of the farmer’s decision to adopt alternative technologies in agriculture. The decision concerns the allocation of lands for old and new technology. We consider the case where organic production requires adoption of a new/alternative technology to be profitable. This paper suggests that an entirely theoretical exercise can illuminate parts of this complex issue which empirical work has not been able to reach. We show the importance of (1) the available quantity of land devoted to agriculture, (2) the productivity of the new/alternative technology, (3) the incentive mechanism and finally, (4) the constraints on output of the new/alternative technology. The results of this article are not confined to the agricultural sector. The insights apply to other technology decisions.

30.

- Title: Demand of farmers in Van Giang, Hung Yen, Viet Nam for education and training husbandry

- Journal: International Journal of Economic Research

- Author from VNUA:Trần Thị Hà Nghĩa

- Authors:

- Abstract:

This survey research was carried out to investigate the demand for husbandry education and training of farmers in Van Giang, Hung Yen, Viet Nam. The result shows that most farmers in Van Giang need more primary training for their husbandry in short time. This study also provides practical suggestions to the design and organization of animal husbandry training programmes for these local people.

31.

- Title: Pileamartines A and B: Alkaloids from Pilea aff. martinii with a new carbon skeleton

- Journal: Tetrahedron Letters

- Author from VNUA:1. Đoàn Thị Thúy Ái 2. Phạm Văn Cường

- Authors: Đoàn Thị Thúy Ái, Trịnh Thị Thanh Vân, Đoàn Thị Mai Hương, Marc Litaudon, Lê Huyền Trâm, Châu Văn Minh, Phạm Văn Cường

- Abstract:

Two novel alkaloids, with an unprecedented carbon skeleton, pileamartines A (1) and B (2), together with the known alkaloid, julandine (I-a) were isolated from the alkaloidal fraction of Pilea aff. martinii leaves. Their structures were established by spectral data analysis, including MS and 2D NMR. The absolute configurations of 1 and 2 were suggested by comparison of their experimental and calculated electronic circulardichroism spectra. Compound 2 exhibited weak inhibitory activity against the a-glucosidase enzyme with an inhibition of 11% at a concentration of 200 µM. These compounds were not cytotoxic

against several cancer cell lines even at a concentration of 150 µM.

32.

- Title: In vitro analysis of DIMBOA catabolism in the Asian corn borer Ostrinia furnacalis (Lepidoptera: Crambidae)

- Journal: Applied Entomology and Zoology Vol 53 , Issue 2 , page 223 - 227

- Author from VNUA:Trần Thị Thu Phương

- Authors: Tran Thi Thu Phuong, Masanobu Yamamoto, Takashi Matsuo, Takeshi Fujii, Yukio Ishikawa

- Abstract:

Larvae of the Asian corn borer Ostrinia furnacalis (Guenée) must cope with 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), a major toxic allelochemical present in its host plant, maize Zea mays L. UDP-glucosyltransferase (UGT), which conjugates glucose to various lipophilic toxic compounds and thereby makes them more hydrophilic for easier excretion, has been suggested to be involved in the detoxification of DIMBOA in several insects. Our previous in vitro assays using O. furnacalis midgut homogenates demonstrated that DIMBOA was catabolized only when UDP-glucose, a glucose donor for UGT activity, was included in the reaction mixture; however, DIMBOA glucoside, the expected product of UGT activity, was only detected in trace amounts in assay products. The present study revealed that DIMBOA glucoside was produced, but was immediately degraded by unidentified enzymes in the midgut homogenate that do not require UDP-glucose for their activities, suggesting the presence of a novel route for DIMBOA catabolism in O. furnacalis.

33.

- Title: Prevalence of Cryptocaryon irritans in wild marine ornamental fish from Vietnam

- Journal: IOP Conf. Ser: Earth Environ.Sci

- Author from VNUA:1. Đoàn Thị Nhinh 2. Kim Văn Vạn

- Authors: Kim Van Van; Doan Thi Nhinh

- Abstract:

Abstract. Wild-caught ornamental marine fish from NhaTrang (Khanh Hoa) and Ha Long (Quang Ninh) in Vietnam were examined over the three sampling batches corresponding to the spring, summer and autumn times for the prevalence of Cryptocaryon irritans. Out of a total of 211 fish (15 species), 143 (67.7 %) were found to be infected with the mean intensity of 7.67 parasites per field of view (x4 magnification). The prevalence of C.irritans in fish caught during the spring (91.0 3% on average) was significantly higher than that of the fish caught during the summer (39.29 %). A wide variation in the prevalence of the parasite was shown among the fish species. The highest prevalence and intensity of the infection occurred in Plataxteira, Diodon holocanthus, Paracanthurus hepatusat 100 % of infection and density of 12 parasites/field of view (x4 magnification) while and the lowest prevalence of C. irritans appeared on Rhinecanthus aculeatus, Zancluscornutus, and Zebrasoma veliferum with less than 50% of fish infected. Clinical signs of fish infected of C.irritans showed such as tiny white spots on skin, gills, and fins; ragged fins, changes in skin colour, cloudy eyes and increase mucus production.

34.

- Title: Efficiency of Different Integrated Agriculture Aquaculture Systems in the Red River Delta of Vietnam

- Journal: MDPI

- Author from VNUA:1. Trần Thị Nắng Thu 2. Nguyễn Văn Hướng 3. Trần Hữu Cường

- Authors: Nguyen Van Huong, Tran Huu Cuong, Tran Thi Nang Thu, Philippe Lebailly

- Abstract:

Integrated Agriculture Aquaculture (IAA) is characteristic with diversity of small-scale production systems in the Red River Delta, Vietnam where most integrated aquaculture systems are closely associated to the VAC model, an ecosystem production that three components: garden (V), pond (A) and livestock pen (C) are integrated. These VAC systems effectively use all the available land, air, water and solar energy resources, and also effectively recycle by-products and waste for providing diversified agricultural products to meet the complex nutritional demands of rural communities. The IAA systems are dynamic, diverse and subject to economic and environmental changes. By investigating 167 aquaculture households, the traditional VAC, New VAC, Animal Fish (AF) and Commercial Fish (FS) systems are identified as four existing IAA systems. This paper presents the main characteristics and economic efficiency of these IAA systems. The study’s results indicate clear evidence that the traditional VAC system and New VAC system are the most efficient and effective models. The findings of this study have shed light on the important role of integrated aquaculture systems to food security and economic development of households and local communities. The VAC systems are likely to propose for improving household food security and developing the local economy.

35.

- Title: New Freshwater Aquaculture Systems in the Red River Delta of Vietnam: Evolution of a Hey Role in Food Systems and Rural Development

- Journal: Polycy studies organization

3. Tạp chí thuộc: Tạp chí KH quốc tế trong Scopus

4. Năm xuất bản: 2018

5. Nơi xuất bản: Mỹ

- Author from VNUA:1. Trần Thị Nắng Thu 2. Trần Hữu Cường

- Authors: Nguyen Van Huong ,Tran Huu Cuong,Tran Thi Nang Thu, and Philippe Lebailly

- Abstract:

Freshwater aquaculture production systems are closely related to integrated Agriculture Aquaculture systems. The systems are tradi–tionally integrated with crop production, horticulture, and livestock husbandry. Improved aquaculture systems are currently able to not only improve the nutrients of local farmers’ diets and economic conditions, but are also able to create employment opportunities and lead to better resource-utilization and rural development. This paper aims to present the changes in food systems affected by the rapidly developing freshwater aquaculture in northern Vietnam. It will shed light on how the aquaculture system play various roles in supporting agrarian livelihoods, their relationship to well-being, and food security using a clear example of smail-scale aquaculture in a province of the Red River Delta. The challenges and opportu-nities presented to small-scale producers and culture systems are assessed, and the likely futureof small-scale freshwater production systems is discussed and forecast.

Combining historical, adaptive, and systematic approaches, the study revealed the features and characteristics of inland aquaculture systems at the household level over a decade of the evolutionary process (1977-2015). By investigating 151 aquaculture households in two representative districts of the region, the three existing sys-tems are identified: VAC system (23%) (whereby V=vuon-gardens (horticulture), A=Ao-ponds (aquaculture) and C=chuong-animail sheds/pens (livestock husbandry)); aquaculture/livestock (AF) sys-tem (43%); and the commercially intensive fish culture (FS) system (34%). Beyond the positive benefits to the food system and rural development, potential considerably exists for further aquaculture integration within VAC systems in the region, which will contrib-ute to poverty reduction and improvement of the livelihoods of small-scale rural farmers.

36.

- Title: Performance appraisals: Obsolete of useful in today's data-driven world

- Journal: Imperial Journal of interdisciplinary Research

- Author from VNUA:Nguyễn Thái Tùng

- Authors: Nguyễn Thái Tùng

- Abstract:

Performance appraisals have undergone radical changes over the last several decades, but a lack of theoretical progress has sparked an academic interest in the theory underlying effective performance appraisals. Many researchers have condemned performance appraisals as destined for collapse and plenty of companies have abandoned their use due to a lack of efficacy in actually motivating employees and evaluating performance. This can be traced back to a lack of training for managers, who struggle to let go of their own biases, but also the actual evaluation criteria. Improving the effectiveness of performance assessments means using objective measures of competency and simplification of existing systems, including more frequent and relevant feedback and coaching that focuses on employees’ strengths for the future.

37.

- Title: Fuzzy Equivalence on Standard and Rough Neutrosophic Sets and Applications to Clustering Analysis

- Journal: Information Systems Design and Intelligent Applications

- Author from VNUA:Nguyễn Xuân Thảo

- Authors: Nguyen Xuan Thao, Le Hoang Son, Bui Cong Cuong, Mumtaz Ali, Luong Hong Lan

- Abstract:

In this paper, we propose the concept of fuzzy equivalence on standard neutrosophic sets and rough standard neutrosophic sets. We also provide some formulas for fuzzy equivalence on standard neutrosophic sets and rough standard neutrosophic sets. We also apply these formulas for cluster analysis. Numerical examples are illustrated.

38.

- Title: Functional roles of root plasticity and its contribution to water uptake and dry matter production of CSSLs with the genetic background of KDML105 under soil moisture fluctuation

- Journal: Plant Production Science

- Author from VNUA:Nguyễn Thị Ngọc Dinh

- Authors: Stella Owusu-Nketia, Jonaliza Lanceras Siangliw, Meechai Siangliw

, Theerayut Toojinda, Apichart Vanavichit, Noppon Ratsameejanphen, Mathurada Ruangsiri

, Sararin Sriwiset, Roel Rodriguez Suralta, Yoshiaki Inukai, Shiro Mitsuya, Mana Kano-Nakata,

- Abstract:

Soil moisture fluctuation (SMF) stress due to erratic rainfall in rainfed lowland (RFL) rice ecosystems negatively affect production. Under such condition, root plasticity is one of the key traits that play important roles for plant adaptation. This study aimed to evaluate root plasticity expression and its functional roles in water uptake, dry matter production and yield under SMF using three chromosome segment substitution lines (CSSLs) with major genetic background of KDML105 and a common substituted segment in chromosome 8. The CSSLs showed greater shoot dry matter production than KDML105 under SMF, which was attributed to the maintenance of stomatal conductance resulting in higher grain yield. The root system development based on total root length of the CSSLs were significantly higher than that of KDML105 due to the promoted production of nodal and lateral roots. These results implied that the common substituted segments in chromosome 8 of the 3 CSSLs may be responsible for the expression of their root plasticity under SMF and contributed to the increase in water uptake and consequently dry matter production and yield. These CSSLs could be used as a good source of genetic material for drought resistance breeding programs targeting rainfed lowland condition with fluctuating soil moisture environments and for further genetic studies to elucidate mechanisms underlying root plasticity.

39.

- Title: Long-time behavior of the one-phase Stefan problem in periodic and random media

- Journal: Discrete and Continuous Dynamical Systems - Series S

- Author from VNUA:Vũ Thị Thu Giang

- Authors: Giang Thi Thu Vu and Norbert Pozar

- Abstract:

We study the long-time behavior of solutions of the one-phase Stefan problem in in homogeneousmedia in dimensions n is greater than or equal 2. Using the technique of rescaling which is consistent with the evolution of the free boundary, we are able to show the homogenization of the free boundary velocity as well as the locally uniform convergence of the rescaled solution to a self-similarsolution of the homogeneous Hele-Shaw problem with a point source. Moreover, by viscosity solution methods, we also deduce that the rescaled free boundary uniformly approaches a sphere with respect to Hausdorff distance.

40.

- Title: Lattice strain in irradiated materials unveils a prevalent defect evolution mechanism

- Journal: PHYSICAL REVIEW MATERIALS

- Author from VNUA:Nguyễn Tiến Hiển

- Authors: Aurélien Debelle, Jean-Paul Crocombette, Alexandre Boulle, Alain Chartier, Thomas Jourdan, Stéphanie Pellegrino, Diana Bachiller-Perea, Denise Carpentier, Jayanth Channagiri, Tien-Hien Nguyen, Frédérico Garrido, and Lionel Thomé

- Abstract:

Modification of materials using ion beams has become a widespread route to improve or design materials for advanced applications, from ion doping for microelectronic devices to emulation of nuclear reactor environments. Yet, despite decades of studies, major issues regarding ion/solid interactions are not solved, one of them being the lattice-strain development process in irradiated crystals. In this work, we address this question using a consistent approach that combines x-ray diffraction (XRD) measurements with both molecular dynamics (MD) and rate equation cluster dynamics (RECD) simulations. We investigate four distinct materials that differ notably in terms of crystalline structure and nature of the atomic bonding. We demonstrate that these materials exhibit a common behavior with respect to the strain development process. In fact, a strain build-up followed by a strain relaxation is observed in the four investigated cases. The strain variation is unambiguously ascribed to a change in the defect configuration, as revealed by MD simulations. Strain development is due to the clustering of interstitial defects into dislocation loops, while the strain release is associated with the disappearance of these loops through their integration into a network of dislocation lines. RECD calculations of strain depth profiles, which are in agreement with experimental data, indicate that the driving force for the change in the defect nature is the defect clustering process. This study paves the way for quantitative predictions of the microstructure changes in irradiated materials.

41.

- Title: Nasal swab - a new tool for the detection of porcine respiratory disease complex in natural infected pigs

- Journal: Acta Scientific microbiology

- Author from VNUA:Nguyễn Thị Trang

- Authors: Nguyen Thi Trang, Pham Hong Ngan, Nguyen Van Hop, Takuya Hirai

- Abstract:

The objectives of the present study were to determine whether nasal swab could be used for the detection of PRDC in naturally infected pigs. Fifteen piglets were used in this study. Serum, oral fluid, nasal swab, lung, tonsil and pulmonary lymph node were collected from each pig. Most common PRDC pathogens are porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), porcine circovirus type 2 (PCV2), Mycoplasma hyopneumonia, Pasteurella multocida, Actinobacillus pleuropneumonia (APP) were examined by nested RT-PCR and multiplex PCR. Among 15 pigs, PRRSV was detected in nasal swabs from 12 pigs, oral fluids from 11 pigs, and serum from only 3 pigs. The ability to detect PRRS virus by nasal swab could be compare with tonsil and lungs which is consider as the first choice of sample. In addition, other pathogens such as PCV2, Mycoplasma and APP could also be detected in nasal swab. As many pathogens cause PRDC in pigs could be detected in nasal swabs, therefore nasal swab could be considered as a preferable sample for the detection of PRDC in pigs. PRRS virus was consistently detected in nasal swab it could be explained by the replication of PRRS virus in lymphoid tissue in the nasal cavity then shed into nasal discharge.

42.

- Title: Quantifying urban growth patterns in Hanoi using landscape expansion modes and time series spatial metrics

- Journal: Plos One

- Author from VNUA:Nông Hữu Dương

- Authors: Duong H. Nong , Christopher A. Lepczyk, Tomoaki Miura, Jefferson M. Fox

- Abstract:

Urbanization has been driven by various social, economic, and political factors around the world for centuries. Because urbanization continues unabated in many places, it is crucial to understand patterns of urbanization and their potential ecological and environmental impacts. Given this need, the objectives of our study were to quantify urban growth rates, growth modes, and resultant changes in the landscape pattern of urbanization in Hanoi, Vietnam from 1993 to 2010 and to evaluate the extent to which the process of urban growth in Hanoi conformed to the diffusion-coalescence theory. We analyzed the spatiotemporal patterns and dynamics of the built-up land in Hanoi using landscape expansion modes, spatial metrics, and a gradient approach. Urbanization was most pronounced in the periods of 2001–2006 and 2006–2010 at a distance of 10 to 35 km around the urban center. Over the 17 year period urban expansion in Hanoi was dominated by infilling and edge expansion growth modes. Our findings support the diffusion-coalescence theory of urbanization. The shift of the urban growth areas over time and the dynamic nature of the spatial metrics revealed important information about our understanding of the urban growth process and cycle. Furthermore, our findings can be used to evaluate urban planning policies and aid in urbanization issues in rapidly urbanizing countries.

43.

- Title: Desalination of brackish water for agriculture: challenges and future perspectives for seawater intrusion areas in Vietnam

- Journal: Journal of Water Supply: Research and Technology

- Author from VNUA:Võ Hữu Công

- Authors: Võ Hữu Công

- Abstract:

This research aims to provide an overview of the seawater encroachment threat on agriculture in lowland areas and potential solutions for better practices. It was found that the Mekong river delta experiences severe impacts from climate change with more than 75% of provinces affected by seawater intrusion, of which Kien Giang, Ca Mau and Ben Tre provinces are the most influenced with 70% affected areas. The salinity of river water was observed in the range of 15–30 g/L in 2015; meanwhile, the strongest tolerated rice species reached ceiling values of 3–4 g/L. Emerging challenges were identified due to the uncertain upstream hydrological regime coupled with high levels of tide, field evaporation and water withdrawal. The development strategies of affected provinces are given on the modification of rice tolerant capacity, and modification to aquaculture in areas with high salinity, in which water purification is in urgent demand. Desalination technologies have been proposed with various innovations which are still not practical on a large scale. The desalination of seawater and brackish water by reverse osmosis, nano-filtration, electro-dialysis, ion-exchange resins, electrochemical processes and thermal distillation has been applied to agriculture. The advance reverse osmosis shows most potential because of its advances in treating performance, cost effectiveness and effective rejection of brine.

44.

- Title: Gender roles in response to water scarcity for coffee production: A case study of an ethnic group in the Central Highland of Vietnam

- Journal: The Social Sciences

- Author from VNUA:Nguyễn Phượng Lê

- Authors: Nguyễn Phượng Lê

- Abstract:

In the Central Highlands, a key coffee growing area of Vietnam, water for coffee production is becoming increasingly scarce. Although central and local governments have implemented several mitigation strategies, only 19 percent of areas under coffee cultivation in the Central Highlands have access to state-constructed irrigation schemes. As a result, coffee producers have had to develop alternative strategies to cope with water scarcity. Based on quantitative and qualitative data collected from an ethic minority group of Ede in the Central Highlands, this research has indicated that farmers develop coping strategies for water scarcity based on their local skills, knowledge, and gender division of labor. Although differentiation of gender roles has been discussed in the literature for agricultural production, few studies have specifically focused on gender roles in construction and implemention of coping strategies in response to water scarcity. It was found that men played more significant roles in responding to water scarcity. However, women’s roles have significantly changed in coffee production in general and in cpoing with water scarcity in particular. To supplement the coping strategies of farmers to water scarcity, it is recommended that central and local governments provide more technical and financial support to male and female farmers.

45.

- Title: Impact of wage employment in agriculture production on labor satisfaction in the Red River Delta Vietnam

- Journal: International Journal of Applied Business and Economic Research

- Author from VNUA:Nguyễn Thị Hải Ninh

- Authors: Nguyen Thi Hai Ninh and Philippe Lebailly

- Abstract:

This paper attempts to identify the impact of wage employment in agricultural production on hired laborerd satisfaction in the Red River Delta of Vietnam. By analysing primary data collected from 150 hired laborers in Bac Ninh, Hai Duong, and Thai Binh provinces, we discover that hired laborers working for farm households are paid both in cash and in kind. During the employed period, hired laborers can receive two forms of wage in cash, daily wage for seasonal workers and monthly wage for long-term workers. Further, hired laborers also receive remuneration in kind such as drink, lunch, bonuses for overtime working, and Lunar New Year gifts. In order to determine factors effecting laborers satisfaction with work, the authors use Exploratory Factor Analysis and the results show that Cronbach’s Alfa coefficient of each group is more than 0.6. That indicates the use of 25 observation variations belonging to 5 factors in the study is suitable. Additionally, logistic model reports that wage, remuneration, working condition, workplace training and gender of employees have a statistically significant effect on job satisfaction of laborers. Of these factors, wage and remuneration report the stronger impact on hired laborers satisfaction. However, hired laborers in farm households state that their wage and remuneration are quite low in comparison with wage of workers in off-farm sectors. Therefore, including regulation on minimum wage and remuneration of hired laborers in agricultural production in to the Labor Law is necessary to enhance hired laborers satisfaction with wage employment.

46.

- Title: Disadvantages and Motivation of Consolidated Financial Statements Preparation in Vietnam

- Journal: International Journal of Economics and Finance

- Author from VNUA:Phí Thị Diễm Hồng

- Authors: Phi Thi Diem Hong, Vu Thi Kim Anh & Manh Dung Tran

- Abstract:

This study is conducted for investigating disadvantages and motivations of consolidated financial statements (CFSs) with illustration of Vietnamese economic groups. As the requirements of international integration process, the international accounting harmonization contributes to do better the financial information presentation, but how to harmonize successfully is question is not easy to be answered. Data were collected from 15 economic

groups in order to have primary data. Then, assumed two key hypotheses to attest the conclusions in early researches. The results show that accounting staffs knowledge as biggest obstacle and legislation as a key motivation of CFSs preparation in Vietnam. The research also finds that the purpose of CFSs is quite difference between different capital owned group types, and the current quality of the CFSs are not still absolutely

convincing the users.

47.

- Title: Treatment of Highly Concentrated Waste Water by Acidification Followed by Ozonation

- Journal: Oil and gas technologies

- Author from VNUA:Đào Thị Thùy Linh

- Authors: Đào Thị Thùy Linh, Ngô Quý Quyền

- Abstract:

The paper presents the results of experimental studies on the possibility of highly concentrated waste water purification by acidification with subsequent ozonation. It was shown that acidification of this waste water to pH = 7 made it possible to destroy the stable initial emulsion, forming aqueous and hydrocarbon phases. At the same time, the degree of water phase purification reached 90.5%. Despite the high efficiency of separation of organic compounds from the studied waste water, the aqueous phase that was obtained after the addition of sulfuric acid to pH = 32222had sufficiently high COD value (36000 mgO /L). The use of the ozonation method at pH = 9 allowed the COD to be reduced up to 9000 mgO /L, while the total purification degree of two stages reached 97.6%.

48.

- Title: The impact of Microfinance on household welfare in rural Vietnam: A case study in Phu Tho province

- Journal: Journal of the Faculty of AgricultureAgriculture, Kyushu university

- Author from VNUA:Đặng Thị Hải Yến

- Authors: DANG Thi Hai Yen, Teruaki NANSEKI, Yosuke CHOMEI

- Abstract:

In Vietnam, microfinance is regarded as one of the important measures to reduce poverty. However, empirical findings indicated that microfinance services can only meet less than half of the poor’s demand. About 3.6 poor and near poor households remained vulnerable to external shocks in 2012. Therefore, this study aims to examine whether and to what extent microfinance programs have helped to increase house- hold economic welfare in rural Vietnam. A survey was conducted in Phu Tho province – one of the poorest areas in the northern of Vietnam through face–to–face structured interviews with 257 respondents. The study employs a two stage least square regression model to deal with self–selection bias in the evaluation of the impact of microfinance on household welfare. The main outcome indicates that formal microfinance has helped to increase the household economic welfare in terms of per capita income, expenditure and value of non–land asset of the household with the coefficients are 0.002, 0.004, 0.003, respectively. Although the effect is small, microfinance has helped the better–off groups to increase their income and expenditure, while the poor groups increase their expenditure and acquire more assets, implying that the credit is distributed to other purpose besides income generating. However, these small impacts suggest a further study about the cost–effective of the microfinance programs.

49.

- Title: Assessment of household risk management strategies for coastal aquaculture: the case of clam farming in Thaibinh Province, Vietnam

- Journal: Aquaculture International Journal

- Author from VNUA:1. Ngô Thị Thu Hằng 2. Trần Hữu Cường

- Authors: Ngô Thị Thu Hằng Hossein Azadi Trần Hữu CườngPhilippe Lebailly

- Abstract:

50.

- Title: Finite Element Simulation on Small Punch Test for Evaluation of J- integral for TRIP Steel

- Journal: Lecture Notes in Mechanical Engineering

- Author from VNUA:Phạm Thị Hằng (KCĐ)

- Authors: Pham Thi Hang, Iwamoto Takeshi

- Abstract:

The small punch test (SPT) has been recently successfully developed for the purpose of evaluate the fracture toughness in not only brittle but also ductile materials. It is considered that fracture toughness of materials can be estimated by means of the SPT based on the measurement of equivalent fracture strain in the SPT and its correlation with fracture toughness. Moreover, fracture toughness of TRIP (transformation-induced plasticity) steel was evaluated by J-integral by using pre-cracked specimen under three-point bending test in the past study. However, the value of J-integral is determined at a limited range of deformation rate in three-point bending test. Thus, fracture toughness of TRIP steel need to be evaluated by means of the SPT, especially at a relatively high deformation rate. Additionally, since the effect of strain-induced martensitic transformation during plastic deformation of TRIP steel coupled with a high increase of temperature is quite complicated, a computational work is indispensable. In this study, finite element simulations are performed for the SPT at various deflection rates and different sizes of specimen and puncher by an application of damage model for type-304 austenitic stainless steel, a kind of TRIP steel. The rate-sensitivity of fracture-mechanical characteristics is examined for different sizes of specimen and puncher. Furthermore, a relationship between equivalent fracture strain in the SPT and J-integral obtained from three-point bending test is challenged to be correlated.

51.

- Title: Tiến tới hệ thống giám sát kháng kháng sinh “Một sức khỏe” tại Việt Nam

- Journal: GREASE

- Author from VNUA:Nguyễn Thị Diễn

- Authors: Marion Bordier Nguyễn Thị Diễn

- Abstract:

Kháng kháng sinh (ABR) là mối đe dọa y tế toàn cầu cần thiết phải thực thi một hệ thống giám sát liên ngành và đa lĩnh vực, gắn liền với khái niệm “Một sức khỏe”. Cộng đồng quốc tế đã ủng hộ mạnh mẽ việc thực thi hệ thống giám sát “Một sức khỏe” trong kháng kháng sinh ở cấp độ quốc gia. Trong bối cảnh đó, chính phủ Việt Nam đã xây dựng chiến lược liên bộ (2013) về kháng kháng sinh, bao gồm một hệ thống giám sát hướng tới các bệnh viện, cộng đồng, vật nuôi làm thực phẩm và thực phẩm bán lẻ. Tuy nhiên, các cơ quan được giao các nhiệm vụ giám sát đang đối mặt với những khó khăn trong việc thực hiện hợp tác liên ngành và đa lĩnh vực được nêu ra ở cấp độ chính sách. Để làm sáng tỏ những nguyên nhân căn bản dẫn đến thành công hay thất bại trong việc hợp tác và đề xuất các giải pháp khắc phục khó khăn, chúng tôi đã tiến hành phỏng vấn các nhóm cơ quan có liên quan trong chiến lược quốc gia này. Chúng tôi đã xác định 7 nhân tố có ảnh hưởng đến sự hợp tác trong cách tiếp cận “Một sức khỏe” đó là: khung quản trị và thực thi chiến lược hiện tại, sự khác biệt về văn hóa của các cơ quan, mức độ hiểu biết, năng lực kỹ thuật, các nguồn lực sẵn có, mâu thuẫn về lợi ích thương mại và sự ảnh hưởng của các đối tác quốc tế. Để khắc phục các trở ngại này, chúng tôi đề xuất một mô hình mới cho quản trị giám sát kháng kháng sinh mà có thể mở đường để tiến tới hệ thống giám sát “Một sức khỏe” hiệu quả và bền vững hơn ở Việt nam.

52.

- Title: Patent protection over traditional knowledge associated with genetic resources in Vietnam: the case of traditional medical knowledge

- Journal: Human and socio-environmental studies

- Author from VNUA:Nguyễn Thị Minh Hạnh

- Authors: Nguyễn Thị Minh Hạnh

- Abstract:

The paper aims at examining how the protection of traditional knowledge associated with genetic resources (ATK) in general and traditional medical knowledge (TMK) in particular under the patent regime has been legally governed and enforced in Vietnam and to what extent such legal framework responses to theoretical and practical challenges associated with TMK protection in the patent law perspective. For those objectives, the paper investigates the related legal documents governing the subject matter with main focus on Law on Intellectual Property Right and Law on Biodiversity in Vietnam, assessing their workability in practice and thereby figuring out the chances and the challenges faced by the system in TMK protection. The paper concludes that the vague and unenforceable provisions, lack of collaboration between patent regime and access and benefit sharing (ABS) framework under the Law on Biodiversity, lack of mechanism for TMK holders to acquire and get benefit from patent right have contributed to the unworkability of the system. The paper also suggests that in the light of experiences from TMK-rich nations, patent rules should move towards the trend that supportively enforces the ABS regime and a patent-adaptive or sui generis framework should be built to achieve more feasible ways of protection if the current patent model fails to protect the legal interests of TMK holders.

53.

- Title: Genetic relationship of asiatic hard clam populations collected in northern coastal provinces in Vietnam based on mtDNA sequence analysis

- Journal: Journal of Aquaculture & Marine Biology

- Author from VNUA:Nguyễn Hữu Đức

- Authors: Vu Thi Trang,1 Le Thi Quynh Chi,3 Chu Chi Thiet,2 Nguyen Huu Duc,3 Tran Thi Thuy Ha1 1Centre of Aquaculture Biotechnology, Research Institute for Aquaculture No.1, Vietnam 2Aquaculture Research Sub-Institute for North Central (ARSINC), Research Institute

- Abstract:

Abstract The genetic relationship of some Asiatic hard clam (Meretrix meretrix) based on mtDNA COI sequence analysis was investigated for populations collected in Thai Binh, Nam Dinh, Nghe An provinces in Vietnam. In addition, this research also targets at species identification based on COI sequences. In total of 59 sequences analyzed, 19 sequences belonged to Meretrix meretrix species with Gen Bank accession number DQ399399.1. 17 sequences of M. meretrix were used for genetic relationship analysis among 3 populations. In which, 6 polymorphic sites, 3 parsimony informative sites and 4 haplotypes observed for the COI gene. Moderately genetic population diversity was observed, overall haplotype and nucleotide diversity were 0.476±0.233 and 0.00151±0.00069, respectively. Generally, genetic differentiation (FST) (FST < 0.15) was moderate. The genetic distance was rather low, which ranged from 0.001 (Thai Binh–NgheAn, Thai Binh–Nam Dinh populations) to 0.002 (Nam Dinh – Nghe An populations). The result of haplotype network constructing indicated that populations shared common haplotype and there was no specific isolation of the haplotypes of the populations. Hence, it showed M. meretrix populations had intimate genetic relationship. The result of phylogenic tree indicated that three M. meretrix populations (Thai Binh, Nam Dinh, Nghe An) had a very small or no genetic variation among populations. Keywords: Population, genetic diversity, genetic relationship, meretrixmertrix, phylogenetic analysis

54.

- Title: Efficiency and adoption of organic tea production: Evidence from Vi Xuyen district, Ha Giang province, Vietnam

- Journal: Asia-Pacific Journal of Regional Science

- Author from VNUA:Tô Thế Nguyên

- Authors: To The Nguyen and Nguyen Anh Tuan

- Abstract:

This paper considers two empirical models. Initially, a stochastic frontier analysis is employed to investigate the technical efficiencies of tea growers in Vi Xuyen, Ha Giang. The findings indicate that the average technical efficiency of organic tea farmers in surveyed area was 65.2% which is lower than that of conventional tea growers (70.1%). Furthermore, the average age of tea plant has negative effect on technical efficiency of tea production, meanwhile, the remaining parameters have positive effects on the technical efficiency. Secondly, binary logistic regression analysis is used to determine the influencing factors of the tea farmer’s choice on organic tea production. The variables such as cultivation land, access to extension services, experience of household’s head and especially contract farming have large influences on the decision of tea farmers. The marginal effects after logit of the explanatory variables are -0.01, 0.63, -0.05, 0.71, respectively.

55.

- Title: Multilocus sequence analysis of Vibrionaceae isolated from farmed amberjack and the development of a multiplex PCR assay for the detection of pathogenic species

- Journal: Journal of Fish Diseases

- Author from VNUA:Trương Đình Hoài

- Authors: Issei Nishiki

Takayuki Minami

Ayana Murakami

Truong Dinh Hoai

Atushi Fujiwara

- Abstract:

Multilocus sequence analysis of Vibrionaceae isolated from farmed amberjack and the development of a multiplex PCR assay for the detection of pathogenic species

56.

- Title: Screening antibacterial effects of Vietnamese plant extracts against pathogens caused Acute Hepatopancreatic Necrosis Disease (AHPND) in shrimps

- Journal: Asian Journal of Pharmaceutical and Clinical Research

- Author from VNUA:1. Nguyễn Thị Thanh Hà ( TY) 2. Nguyễn Thanh Hải (CNSH)

- Authors: Hai Thanh Nguyen, Lua Thi Dang, Hanh Thi Nguyen, Hai Ha Hoang, Ha Thi Ngoc Lai and Ha Thi Thanh Nguyen

- Abstract:

ABSTRACT

Objectives: The objectives are aimed to investigate the antibacterial properties of five Vietnamese medicinal plants against Acute hepatopancreatic necrosis disease (AHPND)-caused bacterial pathogens, in order to verify their potentials to apply as a new treatment therapy.

Methods: Extracts from plants, such as Psidium guajava leaf (P. guajava), Piper betle L. leaf (P. betle), Phyllanthus amarus leaf (P. amanus), Rhodomyrtus tomentosa seed (R. tomentosa) and Allium sativum bulb (A. sativum), were tested against three AHPND-caused bacteria. Agar infusion and broth dilution methods were employed to evaluate extract in vitro antibacterial effects, while experiments with cultured white-leg shrimps were applied to access their safety when applied in vivo. HPLC analysis were applied to identify components in the extracts.

Results: P. amanus and R. tomentosa extracts exerted the strongest inhibition on tested bacteria. Other extracts, including P. betel and P. guajava were less effective, while A. sativum showed no effects against bacteria. In safety assessment experiments, we observed that only crude extracts of R. tomentosa and A. satium were safe, while others significantly reduced their survival rates. HPLC showed that extracts of high antibacterial properties had rich phenol constituents. In addition, phenolic profile of R. tomentosa showed the presence of piceatannol.

Conclusion: Considering both of antibacterial effects and safety properties altogether, we concluded that among the five examined plant materials of this study, R. tomentosa had the highest potential to apply in AHPND treatment, as only this plant showed the high effects on pathogenic bacteria while were still safe for host aquatic shrimps.

Key words: medicinal plant; Rhodomyrtus tomentosa; antibacterial effect; acute hepatopancreatic necrosis disease; AHPND; shrimp.

57.

- Title: Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.

- Journal: Anim Sci J.

- Author from VNUA:1. Nguyễn Văn Thanh 2. Đỗ Thị Kim Lành

- Authors: Thanh-Van Nguyen, Manita Wittayarat, Lanh Thi Kim Do, Thanh Van Nguyen, Masahiro Nii, Zhao Namula, Toshiki Kunihara, Fuminori Tanihara, Maki Hirata, Takeshige Otoi

- Abstract:

Abstract

Electroporation is the technique of choice to introduce an exogenous gene into embryos for transgenic animal production. Although this technique is practical and effective, embryonic damage caused by electroporation treatment remains a major problem. This study was conducted to evaluate the optimal culture system for electroporation-treated porcine embryos by supplementation of chlorogenic acid (CGA), a potent antioxidant, during in vitro oocyte maturation. The oocytes were treated with various concentrations of CGA (0, 10, 50, and 100 μmol/L) through the duration of maturation for 44 hr. The treated oocytes were then fertilized, electroporated at 30 V/mm with five 1 msec unipolar pulses, and subsequently cultured in vitro until development into the blastocyst stage. Without electroporation, the treatment with 50 μmol/L CGA had useful effects on the maturation rate of oocytes, the total cell number, and the apoptotic nucleus indices of blastocysts. When the oocytes were electroporated after in vitro fertilization, the treatment with 50 μmol/L CGA supplementation significantly improved the rate of oocytes that developed into blastocysts and reduced the apoptotic nucleus indices (4.7% and 7.6, respectively) compared with those of the untreated group (1.4% and 13.0, respectively). These results suggested that supplementation with 50 μmol/L CGA during maturation improves porcine embryonic development and quality of electroporation-treated embryos.

KEYWORDS:

antioxidant; chlorogenic acid; electroporation; embryo; oxidative stress

58.

- Title: Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.

- Journal: Cryo Letters.

- Author from VNUA:1. Nguyễn Văn Thanh 2. Đỗ Thị Kim Lành

- Authors: Nguyen TV, Tanihara F, Hirata M, Hirano T, Nishio K, Kim Do LT, Nguyen TV, Nii M, Otoi T.

- Abstract:

BACKGROUND:

Short-term storage is valuable method to reuse manipulated embryos.

OBJECTIVE:

The present study evaluated the effects of antifreeze protein (AFP) supplementation on the quality and development of in vitro-produced porcine morulae after short-term storage (24 h).

MATERIALS AND METHODS:

The morulae were stored with various concentrations of AFP type III for 24 h at 5, 15 and 25C.

RESULTS:

Supplementation of AFP type III (1.0 microgram per mL) improved the developmental competence of embryos stored at 25C. The proportions of DNA-fragmented nuclei in the blastocysts did not differ between the embryos stored at 25C and the control embryos without storage treatment. However, the developmental competence of embryos stored at hypothermic temperatures decreased relative to that of the control embryos.

CONCLUSION:

Supplementation of AFP type III (1.0 microgram per mL) maintained the quality of embryos stored at 25C, but did not have beneficial effects on the development of embryos stored at hypothermic temperatures.

59.

- Title: Quantitative value chain approaches of animal health and food safety

- Journal: Food Microbiology

- Author from VNUA:1. Phạm Văn Hùng 2. Nguyễn Thị Dương Nga 3. Dương Nam Hà

- Authors: (Karl.M.Rich, K. Dizyee, Nguyen Thi Thu Huyen, Duong Nam Ha, Pham Van Hung, Nguyen Thi Duong Nga, Fred Unger, and M.Lucia Lapar)

- Abstract:

Economic impact assessments are increasingly important in the context of animal health and food safety, although much of the existing literature does not address the broader systems context in which disease transmission takes place. In this paper, we discuss the role of system dynamics modeling in addressing the value chain impacts associated with animal health and food safety issues. System dynamics methods hold promise as a means of capturing the complex feedbacks that exist between the biology, economics, and behavioral aspects of food safety and animal health systems. We provide a proof-of-concept of this approach in the context of food safety and animal health in the smallholder pig sector of Viet Nam.

Results highlight the important tradeoffs that exist between policy objectives and the costs required to achieve them.

60.

- Title: Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low fertilization ability in Ban—A native Vietnamese pigs

- Journal: Animal Science Journal

- Author from VNUA:Nguyễn Hoàng Thịnh

- Authors: Nguyen Viet Linh, Tamas Somfai, Thi Hiep Nguyen, Nguyen Thi Nhung, Nguyen Thi Hong, Nguyen Tien Dat, Nguyen Hoang Thinh,

Nguyen Khanh Van, Dong Van Quyen, Hoang Ha Chu, Nguyen Thanh Son, Kazuhiro Kikuchi

- Abstract:

The aim of the present study was to improve the penetration during in vitro fertilization (IVF) of a frozen lot of epididymal sperm with a notoriously low fertilization ability of a Ban boar which is a native Vietnamese breed by optimizing different parameters of the IVF system. In Experiment 1, we determined that Pig-fertilization medium was superior medium to Tyrode’s albumin lactate pyruvate-polyvinyl alcohol medium for IVF and defined the optimum the sperm concentration (1 9 106 sperm/ml). In Experiment 2, we clarified that partial removal of cumulus cells from cumulus-oocyte complexes by hyaluronidase treatment before IVF enhances sperm penetration, whereas complete cumulus removal reduces penetration. Finally, in Experiment 3 the elevation of concentration of caffeine in Pig-fertilization medium from 2 to 5 mmol/L and the prolongation of the co-culture of gametes from 3 to 5 hr significantly increased the total penetration rate from 15.2% to over 50%. In conclusion, the combination of partial oocyte denudation, an elevated caffeine concentration in Pig-fertilization medium and an extended interval of IVF with using an optimized sperm concentration was a potent way to improve the fertilization results

for a frozen epididymal Ban sperm lot with low fertility.

61.

- Title: Sarcomatoid mesothelioma of tunica vaginalis testis in the right scrotum of a dog

- Journal: Journal of Veterinary Medical Science

- Author from VNUA:Nguyễn Vũ Sơn

- Authors: Nguyen Vu Son, James K. Chambers, Takanori Shiga,

Takuya E. Kishimoto, Shotaro Kikuhara, Kohei Saeki, Reina Fujiwara, Masaya Tsuboi, Ryohei Nishimura, Kazuyuki Uchida, Hiroyuki Nakayama

- Abstract:

A 12-year-old intact male Welsh Corgi was presented with enlargement of the right scrotum. Both testicles were surgically removed and histopathologically examined.

On gross examination, white nodules were found in the epididymis and ductus deferens. Histopathologically, the nodules developed continuously from the tunica vaginalis testis of the right scrotum and consisted of spindle-shaped neoplastic cells that invaded the surrounding tissue. Immunohistochemically, the neoplastic cells were di usely positive for vimentin, cytokeratin and Wilms tumor-1 (WT-1). Based on these ndings, the tumor was diagnosed as sarcomatoid mesothelioma. The dog presented with respiratory distress 122 days after surgery and clinical examination found multiple metastatic lesions in the lung, abdominal lymph nodes and peritoneum. The dog died 144 days after surgery due to disease progression.

62.

- Title: Neuromastoma of the hard palate mucosa in an Australian green tree frog (Litoria caerulea)

- Journal: Journal of Veterinary Medical Science

- Author from VNUA:Nguyễn Vũ Sơn

- Authors: Takuya E. Kishimoto, James K. Chambers, Kouki Miyama, Nguyen Vu Son, Mun Keong Kok, Mizuho Uneyama, Yasutsugu Miwa, Hiroyuki Nakayama, Kazuyuki Uchida

- Abstract:

A hard palate mass was surgically removed from an Australian green tree frog (Litoria caerulea) and examined pathologically. The tumor consisted of sheets of small cells arranged in a tubular structure and cords or rosettes with fibrovascular stroma. Immunohistochemically, neoplastic cells were diffusely positive for cytokeratin and neuron-specific enolase and partially positive for S-100 and doublecortin. These findings indicate that the tumor originated from the neuroectodermal tissue. Based on these findings, the tumor was classified as a neuromastoma (neuroepithelioma). Sensory cells located in the hard palate of the frog were considered to be the origin of the tumor. The frog died after going through 3 surgeries and experiencing difficulties closing its mouth.

63.

- Title: An evaluation of fracture properties of type-304 austenitic stainless steel at high deformation rate using the small punch test

- Journal: International Journal of Mechanical Sciences

- Author from VNUA:Phạm Thị Hằng (KCĐ)

- Authors: Hang Thi Pham, Takeshi Iwamoto

- Abstract:

An evaluation of fracture properties of type-304 austenitic stainless steel at high deformation rate using the small punch test

64.

- Title: Free vibration analysis of functionally graded doubly curved shell panels resting on elastic foundation in thermal environment

- Journal: International Journal of Advanced Structural Engineering

- Author from VNUA:Dương Thành Huân

- Authors: Trần Hữu Quốc, Dương Thành Huân, Trần Minh Tú

- Abstract:

Abstract. The purpose of the paper is to develop an analytical investigation on free vibration of a simply-supported functionally-graded (FG) doubly-curved shell panels resting on elastic foundation in thermal environment. Heat conduction and temperature-dependent material properties are both taken into account. The temperature field considered is assumed to be a uniform distribution over the shell surface and varied in the thickness direction only. Material properties are assumed to be temperature-dependent, and graded in the thickness direction according to a simple power law distribution in terms of the volume fractions of the constituents. Based on the first-order shear deformation theory and applying the Hamilton’s principle, governing equations of motion are derived. The results of the study are compared with the available published literature. The numerical results obtained reveal that the material volume fraction index, geometrical parameters and temperature change have significant effects on natural frequencies of the FG doubly-curved shell panels.

Keywords: Functionally-graded materials; Doubly-curved shell panels; Free vibration analysis; First-order shear deformation; Elastic foundation; Thermal environment.

65.

- Title: Endothelium-independent and calcium channel-dependent relaxation of the porcine cerebral artery by different species and strains of turmeric

- Journal: Journal of Traditional and Complementary Medicine

- Author from VNUA:Nguyễn Thị Thanh Hà ( TY)

- Authors: Jesmin Akter, Md Zahorul Islam, Md Amzad Hossain,Shinsuke Kawabata, KensakuTakara, HaThiThanhNguyen, De-Xing Hou,Atsushi Miyamoto

- Abstract:

Objective

To clarify the underlying mechanism of turmeric, which is traditionally used as a medicinal plant for the treatment of cardiovascular disorders,such as hypertension,and palpitations.

Methods

Methanol extracts of different turmeric were used. A tissue-organ-bath system was used to investigate the vasoactive effects of methanol extracts from 5 kinds of turmeric on isolated porcine basilar arteries. The arterial rings were suspended in physiological solution that was maintained at 37°C temperature with a continuous supply of 95% O2 and 5% CO2.

Results

All turmeric extracts (20–800μg/mL) induced concentration-dependent relaxation of the isolated porcine basilar artery pre-contracted with U46619 (1-5×10−9M) in arterial rings with or without endothelium. There were no significant differences in the relaxation induced by different turmeric or between the endothelium-intact and denuded arteries. In depolarized, Ca2+-free medium, the turmeric extracts inhibited CaCl2-induced contractions and caused a concentration-dependent rightward shift of the response curves. In addition, propranolol (anon-specificβ-adrenoceptor antagonist) slightly inhibited the relaxation induced by turmeric. In contrast, Nω-nitro-L-arginine, indomethacin, tetraethylammonium, glibenclamide and 4-aminopyridine did not affect turmeric-induced relaxation.

Conclusion

These results demonstrated that turmeric induced endothelium-independent relaxation of the porcine basilar artery, which may be due to the inhibition of extracellular and intracellular Ca2+ receptors and the partial inhibitionofβ-adrenergic receptors in vascular smooth muscle cells.

66.

- Title: Divergence Measure of Neutrosophic Sets and Applications

- Journal: Neutrosophic Sets and Systems

- Author from VNUA:Nguyễn Xuân Thảo

- Authors: Nguyen Xuan Thao,

Florentin Smarandache

- Abstract:

In this paper, we first propose the concept of divergence measure on neutrosophic sets. We also provide some formulas for the divergence measure for neutrosophic sets. After that, we investigate the properties of proposed neutrosophic divergence measure. Finally, we also apply these formulas in medical problem and the classification

problem

67.

- Title: Some Measures of Picture Fuzzy Sets and Their Application in Multi-attribute Decision Making

- Journal: International Journal of Mathematical Sciences and Computing

- Author from VNUA:1. Nguyễn Văn Định 2. Nguyễn Xuân Thảo

- Authors: Nguyen Van Dinh, Nguyen Xuan Thao

- Abstract:

To measure the difference of two fuzzy sets / intuitionistic sets, we can use the distance measure and dissimilarity measure between fuzzy sets. Characterization of distance/dissimilarity measure between fuzzy sets/intuitionistic fuzzy set is important as it has application in different areas: pattern recognition, image segmentation, and decision making. Picture fuzzy set (PFS) is a generalization of fuzzy set and intuitionistic set, so that it have many application. In this paper, we introduce concepts: difference between PFS-sets, distance measure and dissimilarity measure between picture fuzzy sets, and also provide the formulas for determining these values. We also present an application of dissimilarity measures in multi-attribute decision making.

68.

- Title: A new correlation coefficient of the intuitionistic fuzzy sets and its application

- Journal: Journal of Intelligent & Fuzzy Systems

- Author from VNUA:Nguyễn Xuân Thảo

- Authors: Nguyễn Xuân Thảo

- Abstract:

In this paper, we propose a new correlation coefficient between intuitionistic fuzzy sets. We then use this new result to compute some examples through which we find that it benefits from such an outcome with some well-known results in the literature. As in statistics with real variables, we refer to variance and covariance between two intuitionistic fuzzy sets. Then, we determined the formula for calculating the correlation coefficient based on the variance and covariance of the intuitionistic fuzzy set, the value of this correlation coefficient is in [−1,1]. Then, we develop this direction to build correlation coefficients between the interval-valued intuitionistic fuzzy sets and apply it in the pattern recognition problem.

69.

- Title:浅谈越南北部大众乒乓球俱乐部的类型

- Journal: Tạp chí Khoa học sức khỏe

- Author from VNUA:Đặng Đức Hoàn

- Authors: Đặng Đức Hoàn, Nguyễn Khắc Dược, Nguyễn Văn Hải

- Abstract:

Bóng bàn là môn thể thao được phát triển rộng khắp tại Việt Nam, ngày càng có nhiều người chơi môn thể thao này, vì vậy sự phát triển của các cấp câu lạc bộ cũng phát triển tương đối tốt. Tuy nhiên, hiện nay các cấp câu lạc bộ bóng bàn hoạt động chưa có hiệu quả, cơ cấu quản lý chưa cụ thể, chưa rõ ràng các quy định... cho nên không thể phát huy hết tính tích cực của những người tham gia.

Trước mắt, câu lạc bộ tại các tỉnh phía Bắc Việt Nam có 3 dạng chủ yếu đó là: Do chính phủ lập lên; Chính phủ và tổ chức nhân dân thành lập lên và do cá nhân tự lập lên. Hiện còn rất nhiều bất cập và sau này loại hình cá nhân tự thành lập là chủ yếu.

70.

- Title:浅谈河内师范大学专项学生培养方案及排球课程要点

- Journal: Tạp chí Khoa học sức khỏe

- Author from VNUA:Đặng Đức Hoàn

- Authors: Đặng Đức Hoàn, Nguyễn Văn Hải, Nguyễn Khắc Dược

- Abstract:

Thể thao được coi là một bộ phận quan trọng của giáo dục, rèn luyện thể thao giúp cho sinh viên có thể phát triển toàn diện. Môn học giáo dục thể chất ảnh hưởng trực tiếp đến sự phát triển của sinh viên, ảnh hưởng rõ nhất đó là sự phát dục bình thường của cơ thể, giúp tăng cường thể chất, nâng cao trình độ sức khỏe. Được coi là môn thể thao trọng điểm, bóng chuyền ngày càng được các sinh viên yêu thích. Ngoài ra, bóng chuyền cũng là môn thể thao thi đấu quan trọng của nước nhà. Vì vậy, trong giảng dạy bóng chuyền giáo viên, chỉ đạo viên, huấn luyện viên cần hiểu các góc độ từ kỹ chiến thuật đến các phương pháp huấn luyện của bóng chuyền, có hiểu được sâu xa vấn đề mới có được các khái niệm đúng đắn và từ đó mới phát huy được chất lượng giảng dạy, giúp cho bóng chuyền ngày càng phát triển mạnh hơn.

71.

- Title: Thực trạng công tác giáo dục thể chất tại các trường sư phạm tại Hà Nội

- Journal: Tạp chí Khoa học sức khỏe

- Author from VNUA:Đặng Đức Hoàn

- Authors: Đặng Đức Hoàn, Trần Văn Khôi

- Abstract:

Nâng cao bồi dưỡng phát triển chất lượng của sinh viên là nhiệm vụ chính trị cao cả của các trường đại học, các bộ môn khoa học và trong đó có cả môn học giáo dục thể chất. Để hoàn thành các nhiệm vụ bồi dưỡng giáo dục, các môn khoa học giáo dục tại các trường không ngừng cải tiến về phương pháp và chất lượng. Nghiên cứu cho thấy, nhiều năm nay, khối các trường sư phạm tại hà nội đã thực hiện tương đối tốt các nhiệm vụ giảng dạy, trong đó có cả môn học giáo dục thể chất. Các trường đã tổ chức các hoạt động ngoại khóa nhằm tạo điều kiện tốt cho sinh viên có môi trường để tập luyện, nâng cao hiệu quả công tác giáo dục thể chất. Hiện tại có các câu lạc bộ như: Bóng đá, bóng bàn, cầu lông, võ thuật... Tuy nhiên, các hình thức vận hành các câu lạc bộ chưa có sự thống nhất, mô hình chưa rõ ràng, còn rất nhiều bất cập dẫn đến hiệu quả công tác giáo dục thể chất chưa thực sự tốt.

72.

- Title: High prevalence of antibiotic resistance in Salmonella and Escherichia coli isolated from pig farms and slaughterhouses in North Vietnam

- Journal: Journal of Agriculture and Technology Management (JATM)

- Author from VNUA:1. Chu Thị Thanh Hương 2. Trương Hà Thái 3. Nguyễn Thị Lan (KTY)

- Authors: Truong Ha Thai Pham Thi Ngoc Nguyen Thi Lan Chu Thị Thanh Huong

- Abstract:

This study presents the high prevalence of antibiotic resistance of Salmonella and E. coli among selected pig farms and slaughterhouses in three provinces and two cities in North Vietnam. Salmonella and E. coli were mostly isolated from the sewage, anus feces, caecum, slaughterhouse's floors, animal keeping floors, piggery floors, and carcass samples. The prevalence of Salmonella was 14.0% and 40.9%, and meanwhile, E. coli were 68.2% and 67.2% of the collected samples at the pig farms and slaughterhouses, respectively. Among the 189 Salmonella isolates, 179 isolates were serotypeable belong to 10 serovars. Derby (21.7%) was the most common serovar, followed by Typhimurium (18.5%),Anatum (14.3%), Rissen (12.2%) and London (8.5%). The other serovars were Agona, Weltevreden, Meleagridis, Braenderup, and Chartres. Resistance to streptomycin (81.5% and 78.0%), tetracycline (78.3% and 89.0%), and ampicillin (55.6% and 64.0%) were most commonly observed in Salmonella and E. coli , respectively. Markedly lower resistance rates were observed in both these bacteria for trimethoprim, nalidixic acid, ceftazidime, norfloxacin, nitrofurantoin, ciprofloxacin, and gentamicin. Interestingly, decreased susceptibility to quinolones group (ciprofloxacin, norfloxacin, and nalidixic acid) and third -generation cephalosporins (ceftazidime) was commonly detected in the Salmonella and E. coli isolates. Moreover, resistance rates of the E. coli isolate to trimethoprim, norfloxacin, nalidixic acid, ciprofloxacin, and gentamicin were significantly higher than those in the Salmonella isolates (p<0.05).